bFGF和PDGF对家兔角膜内皮损伤修复作用的研究  

The study of rabbits corneal endothelial wounds healing by bFGF and PDGF

作　　者：杜海涛[1] 傅少颖[1] 崔静[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院眼科,黑龙江哈尔滨150001

出　　处：《眼外伤职业眼病杂志》2007年第12期901-904,共4页Journal of Injuries and Occupational Diseases of the Eye with Ophthalmic Surgeries

摘　　要：目的观察碱性成纤维细胞生长因子(bFGF)与血小板源性生长因子(PDGF)联合应用时对家兔角膜内皮冷冻损伤后修复的作用。方法48只家兔角膜冷冻损伤后随机分为4组,分别为对照组、bFGF组、PDGF组及联合应用bFGF与PDGF组,于不同时间点分别观察角膜内皮细胞数、六边形细胞百分数、角膜厚度、角膜内皮缺损面积。结果bFGF与PDGF联合应用组应用后的角膜内皮细胞数最多、六边形细胞比例最大、角膜厚度最接近正常、角膜内皮缺损面积最小;联合应用组的角膜内皮恢复的速度和质量最高,与其他3组相比差异有统计学意义。结论bFGF与PDGF联合应用时能促进角膜内皮细胞的增生和移行,对加速角膜内皮损伤后的修复具有协同作用。Objective To observe the roles of bFGF and PDGF used together in the healing process of cornea in freeze-injure rabbit eyes. Methods 48 freeze-injure corneas of rabbits were divided into four groups respectively： control-group, bFGF-group, PDGF-group, bFGF and PDGF used together-group. We observed and studied the number of corneal endothelial cell, the areas of corneal endothelial wounds, the thicknesses of cornea for different days. Results There were more corneal endothelial cells for the bFGF and PDGF used together-group than the others. The percentages of hexagonal cells were more higher, and the areas of cornealendothelial wounds were more small. Otherwise the thicknesses of cornea may get close to the normal. The recovery speed and quality of corneal endothelium in the bFGF and PDGF used together-group were not only higher than the control-group, but also higher than bFGF-group or PDGF-group. Conclusion They can be promoted the proliferation and migration of the corneal endothelium when bF- GF and PDGF are used together,and there is the synergistic action for the healing after injury.

关 键 词：角膜内皮 碱性成纤维细胞因子 血小板源性生长因子 

分 类 号：R772.21[医药卫生—眼科]