高粱属A-PAGE图谱的建立及在种子鉴定中的应用  

A-PAGE Establishment in Genus Sorghum and Its Application for Seed Identification

作　　者：方雪恩[1] 陈沁[1] 印丽萍[2] 丁俊波[1] 王伟[1] 

机构地区：[1]上海大学生命科学学院,上海200444 [2]上海出入境检验检疫局,上海200135

出　　处：《西北植物学报》2007年第12期2399-2403,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：上海科委课题(04DZ05003)

摘　　要：以高粱、苏丹草等5种高粱属植物的种子为材料,利用酸性聚丙烯酰胺凝胶电泳(A-PAGE)检测其醇溶蛋白.同时研究了丙酮处理、样品上样量和凝胶浓度对电泳的影响.结果表明:样品经丙酮处理后,在上样量为32μg、凝胶质量分数为15%时电泳可以得到清晰的A-PAGE图谱,据此可以鉴定5种高粱属植物.Prolamins of different genus Sorghum species, such as Sorghum bicolor, Sorghum sudanense, were detected by acid polyacrylamide （A-PAGE） gel electrophoresis. Meanwhile, the effects of acetone, sample volume and gel concentration on electrophoresis were studied. The results showed that clear electrophoresis pattern could be obtained by using 15% A-PAGE with 32μg prolamin after acetone treating. Finally,5 species of Sorghum could be distinguished by the pattern.

关 键 词：高梁属植物 醇溶蛋白 A-PAGE 种子鉴定 

分 类 号：Q789[生物学—分子生物学]