乳腺癌外周血微转移与远处转移的相关性  

Correlation of Peripheral Blood Micrometastasis to Distant Metastasis of Breast Cancer

作　　者：高纪东[1] 王军[2] 张保宁[1] 王翔[1] 梁建明[2] 王靖[1] 毕晓峰[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院腹部外科,北京100021 [2]中国医学科学院中国协和医科大学肿瘤研究所中心实验室,北京100021

出　　处：《癌症》2007年第12期1385-1387,共3页Chinese Journal of Cancer

摘　　要：背景与目的:乳腺癌是一种全身性疾病,早期就可发生血行转移。本研究采用流式细胞仪定量检测乳腺疾病患者外周血肿瘤细胞含量,探讨外周血肿瘤细胞含量与远处脏器转移的相关性。方法:以人细胞角蛋白抗体和CD45抗体双色免疫标记,应用多参数流式细胞仪方法检测65例乳腺癌患者术前和10例乳腺良性病变及8例健康献血者外周血标本中肿瘤细胞含量。结果:65例乳腺癌患者中,57例检测出肿瘤细胞,阳性率为87.7%;乳腺良性病变、健康献血者外周血中未检测到肿瘤细胞。外周血肿瘤细胞阳性率与肿瘤T分期及N分期具有相关性(r=0.271,P=0.017;r=0.393,P=0.002)。除2例失访外,63例患者经过5年随诊,25例出现远处脏器转移,5年转移率为39.7%。所有远处转移病例均为外周血检测阳性患者,8例术前外周血检测阴性的患者均未发现远处转移,两组比较差异有统计学意义(P=0.014);而远处转移与肿瘤T分期、N分期均无明显相关性。结论:术前外周血肿瘤细胞数量与远处转移具有明显相关性,多参数流式细胞术检测乳腺癌患者外周血肿瘤细胞,对指导乳腺癌的个体化治疗具有潜在的应用价值。BACKGROUND ＆ OBJECTIVE： Breast cancer, a whole body disease, can metastasize at early stage. This study was to explore the correlation of peripheral blood cancer cell （PBCC） content to distant metastasis of breast cancer. METHODS： The PBCC content of 65 breast cancer patients and 8 healthy donors was detected by multi-parameter flow cytometry （FCM） with CD45 and cytokeratin staining. RESULTS. Cancer cells were detected in peripheral blood samples from 57 of the 65 patients; the positive rate was 87.7%. No cancer cell was found in peripheral blood samples from healthy donors. The positive rate of PBCCs was correlated to T stage （r=0.271,P=0.017） and N stage （r=0.393, P=0.002）. The patients were followed for 5 years; 2 were lost. Distant metastasis was found in 25 patients with PBCCs. In contrast, no metastasis was found in 8 patients without PBCCs （P〈0.05）. CONCLUSION. Preoperative PBCC content is closely related to distant metastasis of breast cancer. The detection of PBCCs might be useful for individual treatment decision for breast cancer.

关 键 词：乳腺肿瘤 外周血肿瘤细胞含量 流式细胞仪 微转移 

分 类 号：R737.9[医药卫生—肿瘤]