复合稳定剂对碱性蛋白酶的作用研究  

Study on Effects of Compound Stabilizing Agent on the Alkaline Protease Activity

作　　者：郝继兴[1] 殷向彬[1] 刘兴旺[1] 路福平[1] 杜连祥[1] 

机构地区：[1]天津科技大学生物工程学院天津市工业微生物重点实验室,天津300457

出　　处：《化学与生物工程》2007年第12期47-50,共4页Chemistry & Bioengineering

摘　　要：通过单因素实验得出了对碱性蛋白酶稳定效果较好的几种保护剂:5 mmol.L-1Ca2+;20 mg.mL-1微胶粒抑制剂丙二醇-单甲醚;0.01%(质量体积比)可逆蛋白酶活性抑制剂4-甲酰苯基硼酸;1%甘油。在此基础上,进行L18(37)正交实验,以相对酶活率为指标,考察了不同酶活性保护剂在不同条件下对液体碱性蛋白酶活力的影响,筛选到一种优质高效的碱性蛋白酶稳定剂配方为:5 mmol.L-1Ca2+,15 mg.mL-1丙二醇-单甲醚,0.015%4-甲酰苯基硼酸,1%甘油。加入液体碱性蛋白酶40℃保存15 d后,相对酶活力仍保持在85%以上。Some additives that could enhance the enzyme stability were screened through single factor experiments, such as 5 mmol · L^-1 Ca^2＋, 20 mg · mL^-1 trimethylene glycol-single methyl ether, 0. 01% 4-formyl phenyl-boron dihydroxide,1% glycerine. The different enzyme protecting agent had effected on the liquid alkaline protease under the dissimilar condition. Taking the living rate of enzyme as the target, we obtained one high effective alkaline protease stabilizing agent formula through orthogonal experiments as following ： 5 mmol· L^-1 Ca^2＋, 15 mg · mL^-1 trimethylene glycol-single methyl ether, 0. 015% 4-formyl phenyl-boron dihydroxide,1% glycerine. The liquid alkaline protease activity that was joined compound stabilizing agent for 15 days could maintain above 85 % at 40℃.

关 键 词：碱性蛋白酶 复合稳定剂 正交实验 

分 类 号：TQ925.2[轻工技术与工程—发酵工程]