超声波协同丙醇-硫酸铵双水相体系提取灯盏花总黄酮的研究  

Study on Propanol-(NH_4)_2SO_4 Aqueous Two-phase System and Ultrasonic Wave Co-extraction of Total Flavones from Erigeron Breviscapus

作　　者：那吉[1] 董学畅[1] 王雪梅[1] 高云涛[1] 李世月[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《化学与生物工程》2007年第12期51-53,共3页Chemistry & Bioengineering

摘　　要：将超声波技术和双水相萃取技术耦合用于提取灯盏花中总黄酮。通过单因素实验与正交实验得出最佳提取工艺条件为:丙醇体积分数50%、料液比1∶30、超声提取15 min,此时总黄酮平均得率为6.01%。将该方法与常规醇提法进行比较,结果表明两种方法提取率相当,但超声波-双水相提取法快速、简便,提取与分离能同时完成,具有明显的优势。The ultrasonic wave and aqueous two-phase system co-extraction of total flavones from Erigeron breviscapus was studied in the thesis. The optimum extracting conditions were confirmed through the single factor experiment and orthogonal experiment. The best distilled conditions were as follows： the volume fraction of propanol 50%, the ratio of solid to solution 1 ： 30, extraction time 15 min. And the average yield of total flavones achieved 6.01%. A comparison was made between above method and alcohol extraction method. The yield of alcohol extraction was almost the same as that of the other. With shorter reaction time and simpler operation, and it could conduct extraction and separation at the same time, therefore, ultrasonic wave and aqueous twophase extraction was obviously superior to alcohol extraction.

关 键 词：超声波 双水相 灯盏花 总黄酮 协同提取 

分 类 号：TQ028[化学工程] R284[医药卫生—中药学]