奇异变形杆菌TJ-1产絮凝剂的培养基优化研究  

Study on the optimal flocculant-producing culture conditions of Proteus Mirabilis TJ-1

作　　者：杨阿明[1] 夏四清[1] 王学江[1] 张志强[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制及资源化国家重点实验室,上海200092

出　　处：《环境科学学报》2007年第12期1988-1993,共6页Acta Scientiae Circumstantiae

基　　金：2005年度高等学校博士学科点专项科研基金(No20050247016);教育部新世纪优秀人才计划(NoNCET-05-0387)~~

摘　　要：从活性污泥中分离出1株新型高效产絮凝剂菌TJ-1,经16S rDNA相似性分析鉴定为奇异变形杆菌(Proteusmirabilis),这是首次发现奇异变形杆菌能产微生物絮凝剂,对其进行了产絮凝剂培养条件优化研究.结果表明,TJ-1菌产絮凝剂的最佳培养基成分为:葡萄糖10g,蛋白胨1g,MgSO40.3g,KH2PO42g,K2HPO45g,去离子水1L,pH=7.0.絮凝实验结果表明,该菌在最佳培养条件下所产絮凝剂对高岭土悬液的絮凝率达到92%.A new kind of high flocculant - producing strain TJ-1 was obtained from activated sludge. It was identified as Proteus mirabilis by analysis of 16S rDNA similarity. This is the first time to identify Proteus mirabilis strain is able to produce microbial flocculant. The flocculant - producing cultivation conditions were studied and the culture media which optimized production of bioflocculant by strain TJ-1 were：glucose 10 g, peptone 1 g, MgSO40.3 g, KH2PO4 2 g,and K2HPO45 g in 1 L H2O at pH 7. The flocculation test results showed that the flocculating activity towards a kaolin suspension reached 92% when the bioflocculant was produced at the optimal culture conditions.

关 键 词：奇异变形杆菌 生物絮凝剂 培养基 

分 类 号：X172[环境科学与工程—环境科学]