三七总皂苷对异丙肾上腺素所致大鼠心肌肥厚和纤维化的影响  

Protective effects of total saponins of panax notoginseng on myocardial hypertrophy and fibrosis induced by isoproterenol in rats

作　　者：周俐[1] 赵海燕[1] 刘建新[2] 周青[1] 何蔚[2] 

机构地区：[1]赣南医学院机能实验室,江西赣州341000 [2]赣南医学院药理教研室,江西赣州341000

出　　处：《中国病理生理杂志》2007年第12期2346-2349,共4页Chinese Journal of Pathophysiology

摘　　要：目的:研究三七总皂苷(PNS)对异丙肾上腺素所致大鼠心肌肥厚和纤维化的保护作用。方法:用异丙肾上腺素(ISO)5mg·kg-1·d-1,sc,连续7d,建立大鼠心肌肥厚和纤维化模型。造模第2d开始给大鼠腹腔注射PNS25和50mg·kg-1·d-1,连续14d,测定全心重量指数(HW/BW)、左心室重量指数(LVW/BW即LVI);采用试剂盒用分光光度法检测左心室心肌组织中羟脯氨酸(Hyp)、丙二醛(MDA)、一氧化氮(NO)含量和超氧化物歧化酶(SOD)、谷光苷肽过氧化酶(GSH-Px)、一氧化氮合酶(NOS)活性;用放免分析法检测左心室心肌组织中血管紧张素Ⅱ(AngⅡ)含量。结果:ISO模型组大鼠的HW/BW、LVI、左心室HyP、AngⅡ、MDA含量和诱生型NOS(iNOS)活性显著高于生理盐水对照组,SOD、GSH-Px及结构型NOS(cNOS)活性和NO含量明显比生理盐水对照组低;PNS治疗组左心室心肌组织中NO含量、cNOS、SOD和GSH-Px活性明显高于ISO模型组;MDA和AngⅡ含量及iNOS活性和心脏重量指数比ISO模型组低。结论:PNS有抗心肌肥厚和纤维化作用,该作用与清除氧自由基及升高NO含量有关。AIM ： To investigate the protective effects of total saponins of panax notoginseng （PNS） on myocardial hypertrophy and fibrosis induced by isoproterenol （ISO） in rats. METHODS： Myocardial hypertrophy and fibrosis model of rats were induced by injection of ISO （5mg·kg^-1·d^-1, sc） for 7 days. From day 2, the rats were administered with PNS at dose of 25 and 50mg·kg^-1·d^-1, ip for 14 days, the control and ISO model group were received saline injection. Then, the heart - weight （HW） , left ventricular weight （ LVW）, the ratio of HW/BW and LVW/BW （LVI） were measured; the hydroxyproline and malondialdehyde （MDA） and angiotensin （AngⅡ） content of left ventricle. The level of nitric oxide （NO）, nitric oxide synthase （NOS）, superoxide dismutase （SOD） and glutathione peroxidase （GSH- Px） activities in left ventricle were determined by spectropbetemetry and radioimmunoassay, respectively. RESULTS： Compared with NS control group, the ratio of HW/BW, LVW/BW and the content of hydroxyproline, AngⅡ, MDA and iNOS activity in the left ventricle were significantly increased. The cNOS, SOD, GSH- Px activities and NO content were obriously decreased in the ISO model group. After treatment with PNS, the left ventricular NO content, cNOS, SOD and GSH - Px activities were markedly higher than those in ISO model group. The content of MDA, Angll and iNOS activities and the ratio of HW/BW, LVI were significantly lower than those in ISO model group. CONCLUSION ： PNS reverses the my- ocardial hypertrophy and fibrosis induced by isoproterenol in rats. This effect may be related to eliminating the oxygen free radicals and raising NO level.

关 键 词：三七总皂苷 异丙肾上腺素 心肌肥厚 纤维化 氧自由基 一氧化氮 

分 类 号：R363[医药卫生—病理学]