鼻咽癌组织中EB病毒潜伏相关基因的表达  

The latent gene expression of Epstein-Barr virus in nasopharyngeal carcinoma

作　　者：尹志华 蒋卫红[2] 李峰[3] 杨旭宇[3] 冯湘玲[3] 姚开泰[3] 

机构地区：[1]深圳市福田人民医院香蜜湖分院,广东深圳518040 [2]中南大学湘雅医院耳鼻喉科,湖南长沙410078 [3]中南大学肿瘤研究所,湖南长沙410078

出　　处：《中国病理生理杂志》2007年第12期2374-2378,共5页Chinese Journal of Pathophysiology

摘　　要：目的:通过检测鼻咽癌组织中EB病毒的潜伏膜蛋白LMP1的序列以及LMP1、EBNA1、EBNA2的mRNA表达来探讨EB病毒的感染状态及其表达产物与鼻咽癌的关系。方法:应用PCR法检测鼻咽癌组织中LMP1 DNA的存在,并对鼻咽癌来源的LMP1和EB病毒永生化狨猴B淋巴细胞系B95-8来源的LMP1进行测序,比较序列的差异。利用巢式RT-PCR检测鼻咽癌组织中LMP1、EBNA1、EBNA2的mRNA表达。结果:47例鼻咽癌组织均含有LMP1 DNA,所有鼻咽癌来源的LMP1 DNA与B95-8来源的LMP1 DNA序列比较均存在着多个单核苷酸变异,最明显的是XhoⅠ酶切位点的丢失。测序后显示鼻咽癌来源的LMP1DNA有30个核苷酸的丢失。巢式RT-PCR显示LMP1、EBNA1、EBNA2在鼻咽癌中的mRNA表达率分别为76.6%、80.0%和74.5%。其中EB-NA1的表达是由Qp启动的,而B95-8细胞中EBNA1的表达是由Cp启动的。结论:鼻咽癌中EB病毒的作用途径比较复杂,LMP1、EBNA1、EBNA2等潜伏期基因还有早期裂解基因BARF1均可能参与鼻咽癌的发生发展过程。AIM： To examine the latent membrane protein 1 （LMP1） - DNA sequence in nasopharyngeal carcinoma（NPC） and detect mRNA expression of LMP1, EBNA1, EBNA2, and to explore the relationship between EBV infectious status, expression products and NPC carcinogenesis. METHODS： LMP1 DNA was detected in NPC by PCR. Direct sequence was applied to analyze the difference between NPC - LMP1 - DNA and B95 - 8 - LMP1 - DNA. mRNA expressions of LMP1, EBNA1, EBNA2 in NPC were detected by nested RT - PCR. RESULTS： LMP1 DNA existed in all 47 NPC tissues. Several single nucleotide variations were found between NPC - LMP1 - DNA and B95 - 8 - LMPI - DNA. The notable variation was the lost of XhoⅠ restriction site in NPC. Direct sequence showed 30 bp deletion in NPC. The mRNA expressions of LMP1, EBNA1 and EBNA2 in NPC were 76. 6% , 80.0% and 74.5% respectively by nested RT - PCR. The expression of EBNA1 in NPC was promoted by Q promoter while the expression of EBNA1 in B95 - 8 was promoted by C promoter. CONCLUSION： The way of EBV involved in NPC is complex. Latent genes such as LMP1, EBNA1 and EBNA2 as well as early lytic gene BARF1 may all play certain roles in NPC carcinogenesis.

关 键 词：爱泼斯坦巴尔病毒 鼻咽肿瘤 潜伏膜蛋白质1 

分 类 号：R739.63[医药卫生—肿瘤] R373.9[医药卫生—临床医学]