体外诱导大鼠骨髓间充质干细胞表达酪氨酸羟化酶  

In vitro inducing of rat bone marrow mesenchymal stem cells to express tyrosine hydroxylase

作　　者：陈丽[1] 井绪东[1] 何冬梅[1] 张洹[1] 房宝英[1] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2007年第6期562-565,共4页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国务院侨办重点学科建设基金项目(2005)

摘　　要：目的:探讨体外诱导大鼠骨髓间充质干细胞(mesenchymal stem cells,MSCs)表达酪氨酸羟化酶,为帕金森病细胞移植替代治疗提供特异性分化的细胞。方法:在无菌条件下从SD大鼠的股骨中分离骨髓间充质干细胞,体外培养传3代后,用表皮生长因子、碱性成纤维细胞生长因子和抗坏血酸诱导MSCs表达酪氨酸羟化酶。在镜下,观察MSCs在诱导前后的形态变化;用免疫荧光染色检测酪氨酸羟化酶的表达。结果:MSCs经诱导分化后,胞体逐渐变圆,并伸出神经轴突、树突样突起;免疫荧光化学表明细胞酪氨酸羟化酶染色呈强阳性反应。结论:SD大鼠骨髓间充质干细胞在体外能被定向诱导表达酪氨酸羟化酶,有可能为帕金森病治疗提供特异性分化的细胞。Aim：To investigate that mesenchymal stem cells （MSCs） from rat bone marrow were induced to express tyrosine hydroxylase. Methods： MSCs were isolated from rat bone marrow and were induced with epidermal growth factor （ EGF）, basic fibroblast growth factor（bFGF） and ascorbic acid. Tyrosine hydroxylase （TH） in the treated cells was examined by immunocytochemistry. Results： The untreated cells were spindle-shaped adherent cells. After induction, MSCs were getting round and erupted axon and cytodendrite-like fibers. Immunocytochemistry confirmed that these cells were strongly positive for TH. Conclusion：MSCs from rat bone marrow can be induced to express Tyrosine hydroxylase, which provide a possibility for Parkinson＇s disease to be treated with the cells of special differentiation.

关 键 词：骨髓 间充质干细胞 酪氨酸羟化酶 帕金森氏病 

分 类 号：R575[医药卫生—消化系统]