几丁聚糖对镉抑制HepG2细胞DNA修复酶hOGG-1表达的影响  

Effect of Chitosan on Expression of hOGG-1 in Oxidative Damaged HepG2 Cells Induced by Cadmium Chloride

作　　者：谢虹[1] 范冠宇[1] 王家春[1] 吴志刚[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系教育部环境与健康重点实验室,武汉430030

出　　处：《中国预防医学杂志》2007年第6期673-674,共2页Chinese Preventive Medicine

基　　金：国家自然科学基金资助项目(30371197)

摘　　要：目的研究几丁聚糖对氯化镉抑制HepG2细胞DNA氧化损伤修复酶hOGG-1表达的影响。方法在氯化镉染毒HepG2细胞的同时,于培养液中加入不同浓度的几丁聚糖,采用Western blotting法检测氯化镉单独作用以及几丁聚糖和氯化镉联合作用下HepG2细胞DNA氧化损伤修复酶hOGG-1的表达水平。结果①以40μmol/L氯化镉染毒HepG2细胞24h后,细胞内hOGG-1表达显著下降;几丁聚糖对照组与正常对照组相比,hOGG-1表达差异无统计学意义;②不同浓度几丁聚糖和氯化镉联合作用组HepG2细胞hOGG-1的表达水平均较氯化镉单独作用组高,随着几丁聚糖浓度的增加,hOGG-1表达逐渐增强,并且在高剂量几丁聚糖(0.5g/L)和氯化镉联合作用组差异有统计学意义。结论几丁聚糖对氯化镉致HepG2细胞hOGG-1表达水平降低有拮抗作用。Objective To make a further investigation on the effect of ehitsoan on expression of hOGG-1 in cadmium chloride- induced HepG2 cells. Methods HepG2 cells were treated with 40 μmol/L cadmium chloride and ehitosan simultaneously for 24 h. HepG2 cells treated with 40 μmol/L cadmium chloride and 0.50 g/L ehitosan were used as control, respectively. The expression of hOGG-1 was semi-quantitated by western blotting. Results In comparison with normal control, the hOGG-1 content in 40 μmol/L cadmium chloride group decreased significantly. Compared to cadmium chloride-treated group, the hOGG-1 content were much higher in the groups treated with cadmium chloride （40 μmol/L） and ehitosan （0.02, 0.10 and 0.50 g/L, respectively） simultaneously, and when the concentration of ehitosan improved from 0.02 g/L to 0.50 g/L, the hOGG-1 contents increased and significant difference was observedat the concentration of 0.50 g/L.Conclusion This study suggested that the decrease of hOGG-1 content in HepG2 cell induced by cadmium chloride could be antagonized effectively by chitosan.

关 键 词：几丁聚糖 镉 DNA损伤修复酶 WESTERN BLOTTING 

分 类 号：R735.7[医药卫生—肿瘤]