清肺渗湿汤对哮喘大鼠肺组织淋巴细胞上凋亡调控基因Fas/Fas-L表达的影响  

Effect of Qingfeishenshi Decoction on gene expression of modulating apoptosis in lung lymphocyte of rat with asthma

作　　者：宋曦[1] 张有花[2] 

机构地区：[1]河北省邯郸钢铁集团有限责任公司职工医院老年病科,河北邯郸056001 [2]北京中医药大学,2007级博士研究生北京100029

出　　处：《河北中医》2007年第12期1128-1129,1136,共3页Hebei Journal of Traditional Chinese Medicine

基　　金：河北省中医药管理局科学技术研究计划项目(编号:2007213)

摘　　要：目的观察清肺渗湿汤对哮喘大鼠肺组织淋巴细胞上凋亡调控基因Fas/FasL表达的影响。方法将清洁级雄性Wistar大鼠60只随机分为正常对照组、哮喘模型组、地塞米松组、清肺渗湿汤组、麻杏石甘加味汤组。以卵白蛋白致敏、激发的方式建立支气管哮喘急性发作期模型,各组分别给予相应治疗,免疫组化ABC法检测肺组织中Fas/FaL-s的蛋白表达。结果清肺渗湿汤、地塞米松片、麻杏石甘加味汤均能增强哮喘大鼠肺组织中淋巴细胞上促凋亡基因Fas/FaL-s的蛋白表达,与哮喘模型组比较差异有统计学意义(P<0.05),清肺渗湿汤、地塞米松片效果显著,明显优于麻杏石甘加味汤组(P<0.05)。结论通过增强促凋亡基因Fas/Fas-L的蛋白表达而促进炎症局部淋巴细胞的凋亡是清肺渗湿汤减轻哮喘气道炎症反应一个主要机制。objective To observe the effect of Qingfeishenshi Decoction on gene expression of modulating apoptosis in lung lymphocyte of rat with asthma, Methods 60 rats were randomly divided into five groups, they were control group, asthma group, hexadrol group, Qingfeishenshi Decoction group and Mashingshlgan decoction group. Bronchial asthma in acute episode model was established by a manner of egg albumen sensibilization and provocation. Rats in each group were treated by different drugs. Protein expressions of Fas/FaL- s in lung tissue were assayed by immune histochemistry. Results Protein expression of Fas/FaL- s in lung tissue in hexadrol group, Qingfeishenshi Decoction group and Mashingshigan decoction group were potentialized, and were superior to those in control group （ P 〈 0.05）. Protein expression of Fas/FaL- s in lung tissue in hexadrol group and Qingfeishenshi Decoction group were superior to those in Mashingshigan decoction group （ P 〈0. 05）. Conclusions Promoting apoptosis of local lymphocyte by potentiation of gene expression of Fas/FaL- s of modulating apoptosis was important mechanism of Qingfeishenshi Decoction, which can relieve inflammatory reaction of bronchial asthma.

关 键 词：清肺渗湿汤 肺 T淋巴细胞 哮喘 细胞凋亡 基因表达 膜糖蛋白类 生物合成 疾病模型 动物 

分 类 号：R-332[医药卫生] R341