一株产低温碱性蛋白酶海洋细菌Pseudoalteromonas flavipulchra HH407的筛选与生长特性  

Isolation and Physiological Characterization of a Marine Psychrotroph Pseudoalteromonas flavipulchra HH407 Producing Cold-active Alkaline Protease

作　　者：李丹[1,2] 陈丽[3] 李富超[4] 王淑军[3] 李华钟[1,2] 

机构地区：[1]江南大学医药学院,江苏无锡214122 [2]江南大学工业生物技术教育部重点实验室 [3]淮海工学院海洋学院,江苏连云港222005 [4]中国科学院海洋研究所,山东青岛266071

出　　处：《食品与生物技术学报》2007年第6期74-80,共7页Journal of Food Science and Biotechnology

基　　金：国家863计划项目(2006AA02Z153)

摘　　要：对从连云港海域筛选的一株产低温碱性蛋白酶的海洋适冷菌HH407进行的鉴定和生长特性研究结果表明,该菌为革兰氏阴性杆菌,好氧,端生鞭毛,无芽孢及荚膜,大小为0.4～0.7μm×1.0～1.8μm,在酪蛋白培养基的菌落特征为椭圆金黄色、光滑、半透明、四周隆起。根据其生理生化性质、16S rDNA同源性和系统进化树分析初步鉴定该菌属于Pseudoalteromonas flavipul- chra。该菌的生长温度范围为0～40℃,最适生长温度为20℃;pH范围为5.5～11.0,最适生长pH值为8.5;NaCl质量浓度范围为0.5～13 g/dL,最适生长NaCl质量浓度为4 g/dL,无NaCl时不能生长。该菌能较好利用胰蛋白胨、酵母膏和蛋白胨等蛋白质物质,对糖的利用较差。该菌株所产蛋白酶在pH 10.0和35℃时表现最高酶活。A strain H H407 which could production cold-active alkaline protease, was isolated from the sea area of Lianyungang. Then, the identification and physiological characterization of the strain HH407 were studied. The results showed that the strain HH407 was gram negative, rod shaped, aerobic, with a single polar flagellum, neither endospore nor capsule formed, about 0.4 ～0.7 μm×1.0～1.8 μm in size. The colony on screening medium were circular, yellow color, smooth, translucent, convex with regular edges. Based on the 16S rDNA sequence and phylogenetic analysis, as well as the physiological and biochemical tests, the isolate as classified to Pseudoalteromonas flavipulchra HH407. The strain was facultative psychrotrophs by the growth condition test with the optimum growth about 20℃ and grow at 0～40℃. The optimal pH for growth was 8.5 with pH range for growth between pH 5.5 and pH 11. It can grow at a wide range of NaCl concentration of 0. 5～13g/dL with the optimal at 4 g/dL, no growth without NaCl. The protease produced by HH407 exhibited the high activity at pill0.0 and 35℃, respectively.

关 键 词：PSEUDOALTEROMONAS flavipulchra 低温碱性蛋白酶 海洋细菌 筛选 

分 类 号：Q93[生物学—微生物学]