不同宫颈病变中HPV16/18感染与Ki-67表达的关系和临床意义  

Relation between HPV16/18 Infection and the Expression Ki-67 Antigen in Variant Cervical Lesions and Its Clinical Significance

作　　者：李晓玲[1] 邓亚丽[1] 邹明英[1] 董能慧[1] 

机构地区：[1]湘南学院附属医院,湖南郴州423000

出　　处：《湘南学院学报（医学版）》2007年第4期21-23,共3页Journal of Xiangnan University（Medical Sciences）

基　　金：湘南学院科研资助项目(05Y040)

摘　　要：目的探讨在不同宫颈病变中人类乳头状病毒(HPV)16/18感染与Ki-67表达的关系和临床检测的意义。方法应用原位杂交和免疫组化技术对114例不同宫颈病变组织进行HPV16/18型检测和Ki-67抗原表达的观察。结果HPV16/18型阳性者60例(52.63%),其中慢性宫颈炎(CC)9.38%(3/32)、子宫颈尖锐湿疣(CCA)27.27%(6/22)、子宫颈上皮内肿瘤(CIN)78.57%(22/28)、子宫颈浸润癌(ICC)90.06%(29/32);Ki-67抗原阳性表达为61例(53.51%),其中CC12.5%(4/32)、CCA31.82%(7/22)、CIN71.43%(20/28)、ICC93.75%(30/32)。随着宫颈病变的加重HPV16/18阳性率和Ki-67表达率逐渐增高。CC与CCA比较,HPV16/18和Ki-67阳性率均无差异(P>0.05),但良与恶性宫颈病变比较,HPV16/18和Ki-67阳性率均有显著差异(P<0.01)。结论对HPV16/18感染阳性的宫颈病变进行Ki-67检测,在预测宫颈良恶性病变的准确度上有临床指导价值。Objective To explore relation between HPV16/18 infection and the expression Ki-67 antigen in variant cervical lesions and its clinical significance. Methods Situ hybridization and immunohistochemistry methods were used to detect the HPV16/18 infection and expression of Ki - 67 antigen in 114 cases of variant cervical lesions, 32 of chronic cervicitis （CC）, 22 of cervical condyloma acuminate （CCA）, 28 of cervical intraepithelial neoplasm （CIN） and 32 of invasive carcinoma of cervix （ICC）. Results The positive rate of HPV16/18 infection was 52.63%（60/114）. The rate of HPV16/18 infection in CC, CCA, CIN and ICC variant cervical lesions was 9.38 % （3/32）, 27.27 % （6/22）, 78.57 % （2/28） and 90.06 % （29/32）. The positive rate of Ki - 67 was 53. 51%（61/114）. The rate of Ki - 67 in CC, CCA, CIN and ICC variant cervical lesions was 12.5%（4/32）,31. 82% （7/22）, 71.43 % （ 20/28 ）, 93.75 % （30/32）. The positive rates of Ki - 67 and HPV16/18 were increased with cervical lesions severity. The difference of the positive rates of Ki - 67 and HPV16/18 in CC and CCA was not significant（ P 〉 0.05）, While the positive rates of Ki - 67 and HPV 16/18 increased significantly from nonmalignant cervical to malignant cervical （ P 〈 0.05）. HPV16/18 infection was positively correlated with the expression Ki - 67 antigen by correlation analysis （ P 〈 0.05）. Conclusion HPV16/18 infection was positively correlated with the Expression Ki - 67 Antigen. The detect of Ki - 67 maybe play a role in the diagnosis and prediction of cervical lesions in HPV16/18 infection.

关 键 词：宫颈病变 HPV16/18 KI-67 

分 类 号：R711.32[医药卫生—妇产科学]