原发性肝细胞癌患者血清N-糖组的表达分析  

Expression of N-glycome in sera of patients with hepatocellular carcinoma

作　　者：赵云鹏[1] 高春芳[1] 房萌[1] 孙树汉[2] 刘艳[1] 陈冬梅[1] 蔡美琳[1] 

机构地区：[1]第二军医大学东方肝胆外科医院实验诊断科,上海200438 [2]第二军医大学基础部医学遗传学教研室,上海200433

出　　处：《第二军医大学学报》2007年第12期1280-1283,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30571774);上海市科学委员会登山计划(064119522)~~

摘　　要：目的:通过对厚发性肝细胞癌(HCC)与正常人血清N-糖组图谱比较分析,寻找异常峰,为寻求新的HCC肿瘤标志物奠定基础。方法:收集乙型肝炎病毒(HBV)感染的HCC患者(n=100)及健康献血员(n=130)血清,采用基于DNA测序仪的毛细管电泳技术,检测每份标本中荧光标记的N-糖组分。结果:每份血清均得到具有11个峰的图谱,峰值经校正后量化,HCC组较对照组峰1、2、9、11升高,蜂6、8、10降低(P<0.05);且部分峰值变化与肿瘤标志物甲胎蛋白、HBV DNA载量、临床肝功能指标存在相关性。结论:HCC组血清N-糖组图谱较正常对照存在明显的差异,这种变化有望为临床疾病诊断提供依据。Objective.. To compare the expression profiles of serum glycomics between hepatocellular carcinoma（HCC）patients and normal adults, in an attempt to search for new biomarkers for HCC. Methods： Serum samples were obtained from HCC patients with hepatitis B virus（HBV） infection （n= 100） and normal adults（n= 130）. Profiles of serum N-glycome were determined by capillary electrophoresis （CE） based on DNA sequencing equipment. Results： Each sample had a profile with 11 peaks; the peaks were adjusted and quantified. Peaks 1, 2, 9, and 11 in HCC group were higher than those in the control group; peaks 6, 8 and 10 were lower in HCC group than those in control group （P〈0.05）. The changes of peak values of several peaks were correlated with tumor markers such as alpha-fetoprotein（AFP）, HBV DNA copies, albumin and prothrombin time. Conclusion： N-glycome profiles in serum are significantly different between HCC patients and normal adults, which may provide evidence for clinical diagnosis of HCC.

关 键 词：N-糖组 癌 肝细胞 电泳 毛细管 

分 类 号：R735.7[医药卫生—肿瘤]