神经行为学实验Narrow-Alley Test及Corner Test改良:Narrow-Alley Corner Test  

A Modified Experimental Neurological Behavioral Test:the Narrow-Alley Corner Test

作　　者：陈绍春[1] 潘伟生[2] 李明[1] 路钢[2] 冯忠堂[3] 李圆圆[3] 

机构地区：[1]昆明医学院解剖教研室,昆明650031 [2]香港中文大学威尔斯亲王医院神经外科 [3]昆明医学院第一附属医院神经外科,昆明650032

出　　处：《中国实验动物学报》2007年第6期406-409,I0002,共5页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然基金课题资助(编号:30660050)

摘　　要：目的改良传统的Narrow-alley Test及Corner Test,增强实验的可操作性和结果的可靠性。方法用Narrow-alley Test、Corner Test及改良后的Narrow-alley Corner Test检测三组SD大鼠:脑出血+GCSF治疗组;脑出血+生理盐水安慰治疗组;正常对照组。结果(1)与Narrow-alley Test及Corner Test结果一致,正常大鼠在Narrow-alleyCorner Test实验装置中向左、向右"站立转身"的几率接近,而脑损伤大鼠则趋向沿损伤同侧作"站立转身"。(2)Narrow-alley Corner Test不须反复刺激大鼠,减少了人在实验现场对动物活动的影响,增强了实验的可操作性和结果的可信度。结论Narrow-alley Corner Test是一种可操作性强、结果可靠的神经行为学检测方法。Objective To develop a more reliable and feasible behavioral test of experimental neurology by modifying the traditional narrow-alley test and corner test. Method To detect 3 groups of rats with the narrow-alley test, corner test and the narrow-alley corner test. The first group was ICH-GCSF-GROUP in which the rats suffered intracerebral hemorrhage （ICH） operation then were treated with granulocyte colony stimulating factor （GCSF）. The second group was ICH-SALINE-GROUP in which the rats suffered ICH operation then were given vehicle treatment with saline; The third group was SHAM-OPERATED-GROUP in which the rats were only given a sham operation. Result （ 1 ） The same as the narrow-alley test and corner test, the rats in the SHAM-OPERATED-GROUP turned along left or right side in the same rate in the new testing apparatus, but ICH-GROUP of rats tended to turn along the operated side. （2） The testing rats need to be less stimulated in the narrow-alley corner test and the interference of people to the rats was decreased. Conclution The narrow-alley-corner test is a reliable and feasible behavioral test in experimental neurology.

关 键 词：神经行为学 动物实验 改良 

分 类 号：R743.34[医药卫生—神经病学与精神病学]