外源性双歧杆菌对肠外营养幼兔肠道益生菌的影响  

EFFECT OF EXOGENOUS BIFIDOBACTERIA ON THE INTESTINAL PROBIOTICS OF INFANT RABBITS RECEIVING PARENTERAL NUTRITION

作　　者：王翔[1] 陈津津[2] 洪莉[2] 吴江[2] 陶晔璇[2] 蔡威[2] 

机构地区：[1]浙江大学医学院附属儿童医院普外科,杭州310003 [2]上海交通大学医学院附属新华医院临床营养中心,上海200092

出　　处：《营养学报》2007年第6期573-577,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.30271350);"十五"国家科技攻关项目(No.2004BA709B09)

摘　　要：目的:研究外源性双歧杆菌对肠外营养(PN)幼兔肠道益生菌的影响。方法:生后2w的新西兰种白兔24只,分成对照组(n=7)、PN组(n=9)和PN+双歧组(n=8)。对照组为兔食喂养,PN组幼兔完全肠外营养,PN+双歧组幼兔口服青春双歧杆菌0.5×108/d,10d后自直肠取出新鲜粪便。应用SYBR Green I Real-time PCR法,定量检测粪便中双歧杆菌和乳杆菌含量。结果:Real-time PCR法的检测限最低为102PFU,凝胶电泳证实合成探针具有属特异性。粪便细菌定量结果显示,PN组幼兔湿粪(0.05g)中双歧杆菌和乳杆菌含量(对数值)分别为4.62±0.24和4.29±0.49,显著低于对照组(分别为5.84±0.92、5.14±1.07log)和双歧组(6.41±0.39,5.46±0.61log)。结论:SYBR Green I Real-time PCR法能够准确、迅速地对幼兔粪便双歧杆菌和乳杆菌进行定量检测。PN幼兔口服双歧杆菌后,其粪便中双歧杆菌和乳杆菌含量显著增加。Objective： To study the effect of exogenous Bifidobacteria on the intestinal probiotics in the infant rabbits receiving parenteral nutrition（PN）. Method： Two-week-old New Zealand white rabbits weighing 200-250g were divided into three experimental groups： （1） Control group （n=7）; （2） PN group （n=9）; （3） PN＋Bif group （n=8）. PN＋Bif rabbits were gavaged solution of bifidobacteria （0.5× 10^8/d） through oral-stomach catheter. At D10 whole bacteria DNA was extracted from the feces. Genus-specific primers of Bifidobacterium and Lactobacillius were synthesized and their specificities were evaluated by conventional PCR. Real-time PCR was applied to determine Bifidobacteria and Lactobacilli in the feces. Results： The detection limit of the method used in this study was 102 PFU per PCR. Those two kind of genus-specific primers were proved specific by gel electrophoresis very well. The population of Bifidobacteria and Lactobacilli in wet feces （0.05g） of PN rabbits were 4.62±0.24 and 4.29±0.49 log respectively, significantly lower than that of control group （5.84±0.92, 5.14±1.07 log） and PN＋Bif group （6.41±0.39,5.46±0.61 log）. Conclusion： The amount of Bifidobacteria and Lactobacilli significantly increased in the feces of PN infant rabbits receiving exogenous Bifidobacteria.

关 键 词：双歧杆菌 乳杆菌 REAL-TIME PCR 肠外营养 

分 类 号：R459.3[医药卫生—治疗学]