绿茶和红茶多酚对大鼠脂肪分化相关基因表达影响的比较研究  

THE COMPARATIVE STUDY ON EFFECTS OF GREAN TEA AND BLACK TEA POLYPHENOLS ON GENES RELATED TO ADIPOCYTE DIFFERENTIATION IN RATS

作　　者：易娟[1] 邓慧君[2] 曹进[2] 

机构地区：[1]湖南省长沙市卫生学校,长沙410100 [2]中南大学湘雅医学院临床药理研究所茶与健康研究室,长沙410078

出　　处：《营养学报》2007年第6期582-586,共5页Acta Nutrimenta Sinica

摘　　要：目的:比较绿茶多酚(GTP)和红茶多酚(BTP)抗肥胖作用及其分子机制。方法:将实验大鼠分为空白对照组,高脂饲料+GTP组,高脂饲料+BTP组,高脂饲料组,定期检测体重,喂饲3个月后,观察脂肪组织和血脂水平变化,并提取附睾脂肪组织,应用RT-PCR技术观察与脂肪细胞分化相关的基因pref-1,aP2,TNF-α,瘦素,PPAR-γ,C/EBP-α在mRNA水平的表达。结果:GTP和BTP均能明显降低体重,减少脂肪。同时,GTP和BTP均能显著地抑制脂肪细胞特殊标记物aP2,TNF-α,瘦素,但两者之间无统计学差异。此外,GTP还上调前脂肪细胞标记物pref-1,且下调转录因子PPAR-γ。结论:GTP和BTP均可通过调节与脂肪细胞分化相关的基因,逆转脂肪细胞的分化,达到抗肥胖的作用,且GTP抗肥胖作用强于BTP。Objective： To compare the anti-obesity effect of green tea and black tea polyphenols and investigate their molecular mechanisms. Method： Rats were divided randomly into four groups： control group, high-fat group, high-fat diet with green tea polyphenols （GTP） supplement group, and high-fat diet with black tea polyphenols （BTP） supplement group. Body weight was determined every 2 w. After 3 months, the changes of epididymal fat tissues weight and serum lipids were observed. Expressions of those genes associated with adipocyte differentiation in epididymal fat tissues of rats were measured by real-time transcriptionolymerase chain reaction, including pref-γ,aP2,TNF-α, leptin, PPAR-γ, C/EBP-α. Results： Both GTP and BTP prevented the increase of body weight and fat induced by high-fat diet and profoundly down-regulated those adipocyte-specific genes, including aP2, TNF-α and leptin. In addition, GTP also up-regulated the pre-adipocye marker--pref-1 and reduced the expression of transcription factor, peroxisome proliferators-activated receptor （PPAR-γ）. Conclusion： Tea polyphenols could prevent obesity by reversing the adipocyte differentiation, and GTP possessed stronger inhibitory effect than BTP.

关 键 词：绿茶多酚 红茶多酚 肥胖 脂肪细胞分化 

分 类 号：R285.5[医药卫生—中药学]