BMP2真核表达载体的构建  

Construction of BMP2 gene mammal expression plasmid

作　　者：付春江[1] 毕郑钢[1] 黄昀[2] 曲延隆[1] 

机构地区：[1]哈尔滨医科大学第一临床医学院骨外科,黑龙江哈尔滨150001 [2]哈尔滨医科大学医学遗传学教研室,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2007年第6期524-526,共3页Journal of Harbin Medical University

基　　金：黑龙江科技攻关项目(GB01C123-01)

摘　　要：目的为研究骨缺损的基因治疗提供实验基础,构建人骨形态发生蛋白2真核表达载体。方法采用双酶切方法克隆载体将骨形态发生蛋白2基因与真核表达载体pcDNA3.1连接,构建pcDNA3.1-BMP2表达基因载体。结果通过测序分析证实,成功构建BMP2真核表达载体。结论成功构建BMP2表达载体。Objective To clone and construct mammal expression vector for human bone morphogenetic protein 2 （BMP2）,in order to study gene .therapy for bone defect disease.Methods Plasmid pSPBMP2 was digested by Xba Ⅰ and Sal Ⅰ , the BMP2 gene was subcloned into pcDNA3.1. Results The mammal expression plasmid for BMP2, pcDNA3.1-BMP2, had been constructed. The cloned DNA BMP2 gene was confirmed by sequencing. Conclusion The mammal expression plasmid for BMP2 had been successfully constructed.

关 键 词：骨形态发生蛋白-2 基因 基因载体 

分 类 号：R394.3[医药卫生—医学遗传学]