hLRH-1调控结肠腺癌细胞SW480增殖的分子机制  

Molecular Mechanism of hLRH-1 in Regulating Proliferation of Colon Adenocarcinoma Cells SW480

作　　者：郑英梦[1] 王水良[1] 兰风华[1] 

机构地区：[1]南京军区福州总医院检验医学研究所分子医学研究中心

出　　处：《肿瘤防治研究》2007年第12期904-907,共4页Cancer Research on Prevention and Treatment

基　　金：福建省青年科技人才创新资助项目(2004J067);福建省自然科学基金计划资助项目(C0710038);福州总医院院立课题资助项目(200638)

摘　　要：目的初步探讨人核受体hLRH-1在结肠癌发生发展中可能的生物学功能。方法真核表达质粒pcDNA3-hLRH-1经脂质体介导转入SW480细胞中,RT-PCR和Western blot法分别检测外源基因mRNA及蛋白水平的表达;MTT法分析hLRH-1过表达对SW480细胞生长增殖的影响,同时行实时定量RT-PCR法分析细胞生长增殖相关基因Cyclin E1和Cyclin D1、以及凋亡调控相关基因Pten和Rb1的表达。结果hLRH-1过表达的SW480细胞增殖速度明显加快,同时其Cyclin E1基因的表达显著增高,而Pten和Rb1在hLRH-1过表达的SW480细胞中均呈明显的表达下调。结论外源hLRH-1的表达既通过上调Cyclin E1基因的表达而促进SW480细胞增殖,还可能通过调节Pten和Rb1基因的表达而参与细胞凋亡的调控。Objective To explore the possible biological function of human nuclear receptor hLRH-1 in tumorigenesis and progress of colon cancer. Methods Plasmids pcDNA3-hLRH-1 were introduced into SW480 cells via lipofectamine. The expression of mRNA and protein of exogenous hLRH-1 was detected by RT-PCR and Western blotting, respectively. MTT assay was carried out to survey the proliferation of SW480 cells with overexpression of hLRH-1. Meanwhile, the expression of proliferation-related genes cyclin E1 and cyclin D1, and apoptosis-related genes PTEN and Rbl, were analyzed by real-time RT- PCR. Results The proliferation of SW480 cells were promoted under the condition of overexpression of hLRH-1. The expression of cyclin E1 was up-regulated significantly, while that of Pten and Rbl was down-regulated in SW480 cells with overexpressed hLRH-1. Conclusion The expression of exogenous hLRH-1 in SW480 cells induced the proliferation resulting form up-regulation of cyclin El, as well as participated in the regulation of apoptosis via influencing the expression of Pten and Rbl.

关 键 词：核受体hLRH-1 结肠癌 外源基因表达 增殖 

分 类 号：R735.35[医药卫生—肿瘤]