高效液相色谱/挥发激光散射检测器测定奶粉和鸟粪中的甾体化合物  

Determination of Steroids in Milk Powder and Birds' Feces by High Performance Liquid Chromatography Coupled with Evaporative Light Scattering Detector

作　　者：冯埃生[1] 邹汉法[1] 张冰[1] 汪海林[1] 张玉奎[1] 

机构地区：[1]中国科学院大连化学物理研究所国家色谱研究分析中心,大连116011

出　　处：《色谱》1997年第2期102-105,共4页Chinese Journal of Chromatography

摘　　要：利用反相高效液相色谱／挥发激光散射检测器发展了分析甾体化合物的新方法，采用甲醇和水溶液为冲洗体系，梯度淋洗，12种甾体类化合物在35min内得到很好的分离。方法简单快速，不需衍生，样品经固相萃取预处理，回收率介于84.0％～102.8％之间，响应值和浓度不呈简单的线性关系，在5～50mg／L浓度范围内对峰面积与浓度进行对数线性回归分析，得回归系数大于0.99。In this paper, a new, simple and rapid method for the analysis of steroids by reversed-phase high per formance liquid chromatography (HPLC) coupled with evaporative light scattering detector(ELSD) is presented' A good resolution of the 12 steroids is obtained within 35 min with a gradient elution using a binary mobile phase composed of methanol and water. The analytical column used was 2.0mm ×150mm, packed with BDS (particle size 5pm) and the mobile phase flow rate was 0. 2mL/min. Nebulizer gas flow rate and drift tube temperature, the two critical parameters for determining the reproducibility and signal-to-noise ratio of ELSD were 2.00mL/min and 95C l respectively. The response of the ELSD was non-linear as a result of basic light-scattering principles, the calibration curve by plotting lnY vs lnX (Y, X stand for peak area and concentration, respectively) shows a good linearity in the range of 5-50mg/L with a correlation coefficient higher than 0. 99 for individual steroid and with relative standard deviation less than 6. 92 %. This method has been applied to the determination of steroids in milk powder and birds' feces, solid phase extraction on reverse phase bonded C18 (5mL/500mg) was used to treat the samples efficiently. The recoveries of the steroids were between 84. 0%-102. 8% .

关 键 词：高效液相色谱法 奶粉 鸟粪 甾体化合物 ELSD 

分 类 号：O629.2[理学—有机化学] O657.72[理学—化学]