杉木针叶磷脂酰甘油脂肪酸组成分析  

Gas Chromatographic Analysis of Fatty Acids in Phosphatidylglycerol from Needles of Two Crosses of Chinese Fir

作　　者：包宏[1] 李梅[1] 胡兹苓[1] 何祯祥[1] 施季森[1] 

机构地区：[1]南京林业大学,南京210037

出　　处：《色谱》1997年第2期125-126,共2页Chinese Journal of Chromatography

基　　金：国家自然科学基金!39470574;国家"八五"科技攻关项目!85-018-0101

摘　　要：用改进的DEAE阴离子交换柱色谱法和硅胶薄层色谱法分离制备杉木针叶磷脂酸甘油，用气相色谱法测定了它的脂肪酸组成。分析结果表明，抗寒性强的杉木品种的针叶磷脂酰甘油脂肪酸的（18:1＋18:2＋18:3）／[16:0＋16:1（3t）]比值比不抗寒品种的高。This paper reports a method for determination of fatty acids in phosphatidylglycerol from needles of two crosses of Chinese Fir with different cold-resistance. Some simplifications and improvements for analytical method of DEAE CL-6B column chromatography and of silica thin layer chromatography were also discussed' After being extracted with chloroform and methanol, phosphatidylglycerol was isolated from the general lipid extract by using DEAE CL-6B IEC and Silica TLC. Quantitative analysis was conducted by gas chromatography on a DB-FFAP (30m× 0. 32mm i. d. ) column. The results showed that there was a difference in the ratio of fatty acid (18: 1+ 18: 2+18: 3)/[16: 0+16: 1 (3t)] between the two crosses of Chinese Fir with different cold-resistance, 1. 12 for that with higher cold-resistance and 1. 25 for that with lower cold-resistance. A special fatty acid 16: 1(3t) was found in both of the two crosses.

关 键 词：气相色谱法 磷脂酰甘油 脂肪酸 杉木 抗寒品种 

分 类 号：S791.270.1[农业科学—林木遗传育种] O657.71[农业科学—林学]