毛细管胶束电动色谱法分析PTH氨基酸  

Analysis of PTH Amino Acids by Micellar Electrokinetic Capillary Chromatography

作　　者：周建忠[1] 廖杰[1] 钱小红[1] 董芳霆[1] 

机构地区：[1]军事医学科学院国家生物医学分析中心,北京100850

出　　处：《色谱》1997年第2期159-160,共2页Chinese Journal of Chromatography

摘　　要：建立了用毛细管胶束电动色谱法（MEKC）分离19种PTH氨基酸的方法，并探讨了电压、pH值、温度、胶束浓度对氨基酸迁移时间的影响。方法具有速度快、灵敏度高、样品用量少的优点。A useful method for separation of 19 PTH amino acids by micellar electrokinetic capillary chromatography has been developed. Experiments were performed at 35 C with 14kV power, 50μm×57cm fusedsilica capillary column and UV absorption detector. Each new capillary was washed with 0. 1mol/L NaOH and deionized water, each for 15min, be fore use. Samples were injected into the capillary by applying pressure(3402Pa) for 5s. Absorption detection was at 200nm. The running buffer was made up of 50mmol/L sodium dodecyl sulfate(SDS), 50mmol/L sodium of phosphate and borate(pH 7.0). The infulence of operation voltage, pH of buffer, temperature and SDS concentration on the amino acids migration has been studied. The coefficients of variaton of migration time and peak area of 19 PTH amino acids were below 1.0% and 5. 0% respectively. The detection limits were 0. 1-0. 25pmol.

关 键 词：PTH氨基酸 MEKC 氨基酸 

分 类 号：Q517.03[生物学—生物化学] O657.8[理学—分析化学]