高效液相色谱法同时测定血清中茶碱、苯妥英钠、苯巴比妥及卡马西平的药物浓度  

Simultaneous Analysis of Theophylline,Phenobarbital,Amobarbital and Carbamazepine in Serum by High Performance Liquid Chromatography

作　　者：付世江[1] 段盛慧[1] 任群翔[2] 

机构地区：[1]中国医科大学,沈阳110031 [2]沈阳医学院,沈阳110001

出　　处：《色谱》1997年第2期178-179,共2页Chinese Journal of Chromatography

摘　　要：报道了用高效液相色谱法（HPLC）同时测定血清中茶减、苯妥莫纳、苯巴比妥及卡马西平的药物浓度。实验条件:Nova-PahC18柱，流动相为甲醇-水（1:1，V／V），检测波长210nm，流速为1mL／min，萃取液为级访-异丙醇（95:5，V／V）。方法具有灵敏（10-9）、准确（回收率在97％～105％之间）、快速（7min）等特点，对临床血药浓度监测有实际应用价值。Theophylline,phenobarbital,amobarbital and carbamazepine are the chief therapeutic drugs in clinical practice. Because of the differences among inter-individuals in the metabolic clearance of these drugs and their toxicity at certain levels of concentration in serum,the dosage should be regulated to maintain a therapeutic blood drugs level. To achieve this a rapid and accurate assay methed for drugs in blood is necessary. This paper reports that a reversed phase high performance liquid ch romatographic (HPLC) method had been established for the simultaneous separation and quantitative determination of the four drugs in serum.The separation was performed on RP-C18 column using methanol:water(1:1) as mobile phase and 4-aminoantipyrime as internal standard. The eluted substances were detected at 210nm.

关 键 词：高效液相色谱法 茶碱 苯妥英钠 苯巴比妥 血清 

分 类 号：R914.1[医药卫生—药物化学] O657.72[医药卫生—药学]