蛋白质组学技术对肝癌细胞株HepG2的G_2/M期细胞的热激蛋白的鉴定  

ANALYSIS AND IDENTIFICATION OF HEAT SHOCK PROTEIN IN G_2/M PHASE FROM HEPG2 USING PROTEOMICS TECHNIQUE

作　　者：白瑞霞[1] 王文礼[1] 李丽梅[1] 刘婷[1] 

机构地区：[1]内蒙古医学院生物化学与分子生物学教研室,内蒙古呼和浩特010059

出　　处：《内蒙古医学院学报》2007年第5期332-334,共3页Acta Academiae Medicinae Neimongol

基　　金：内蒙古医学院重大科研项目(ny2004-zd-002)

摘　　要：目的:采用蛋白质组学技术鉴定肝癌细胞株HepG2的G2/M期细胞内的热激蛋白。方法:提取肝癌细胞株HepG2的G2/M期细胞的蛋白质,采用双向电泳的方法分离蛋白质,应用图像扫描仪及质谱(MALDI-TOF-MS)分析鉴定HepG2的G2/M期细胞表达的蛋白质。结果:采用双向电泳技术分离,并用质谱成功鉴定出HepG2的G2/M期细胞的热激蛋白。结论:肝癌细胞株HepG2的G2/M期细胞内的热激蛋白的成功鉴定,为探讨HSP在细胞生长和增殖中的作用奠定了基础,并为临床上肿瘤的治疗提供参考依据。Objective：To identify heat shock protein in G2/M phase from HepG2 using proteomics technique. Methods： Proteins of G2/M phase were prepared from human hepatocarcinoma cells and separated using technique of two - dimensional electrophoresis, then analysed and identified via imagine scanner and MALDI - TOF - MS. Results： Heat shock protein in G2/M phase from HepG2 was successfully separated and identified using technique of two - dimensional electrophoresis and MALDI - TOF - MS. Conclusion：The identification of heat shock protein in G2/M phase from HepG2 is laid the foundation of research on HSP in the cell growth and proliferation . It also provides the basis for the treatment of tumor.

关 键 词：肝癌细胞株HEPG2 蛋白质组学 热激蛋白 

分 类 号：Q51[生物学—生物化学] R735.7[医药卫生—肿瘤]