错配修复蛋白hMSH2 hMLH1表达在胃癌发生中的作用及临床意义  

The Role of hMSH2 and hMLH1 Protein Expression in Gastric Carcinogenesis and Its Clinical Significance

作　　者：李梅[1] 刘丽娜[2] 王朝晖[1] 王丽宏[1] 刘志敏[1] 许国旺[3] 吕申[1] 

机构地区：[1]大连医科大学附属第二医院实验中心,辽宁省大连市116027 [2]大连医科大学第一医院消化内科 [3]中国科学院大连化学物理研究所

出　　处：《中国肿瘤临床》2007年第24期1396-1399,共4页Chinese Journal of Clinical Oncology

基　　金：中国科学院知识创新工程领域前沿基金项目资助(编号:DICPkaaaabc)

摘　　要：目的:探讨错配修复蛋白hMSH2、hMLH1表达及其在细胞内表达位置对胃癌发生的影响及临床意义。方法:收集大连医科大学附属第一医院胃癌标本172例、癌旁胃粘膜标本151例,非癌患者胃粘膜标本34例,应用免疫组织化学方法检测每例胃粘膜标本hMSH2、hMLH1蛋白的表达情况(包括其在细胞内表达位置),应用χ2检验,Spearman等级相关分析在SPSS13.0统计软件上作相关统计。结果:胃癌、癌旁胃粘膜和非癌患者胃粘膜hMSH2蛋白表达率分别为69.8%(120/172)、49.7%(75/151)和32.4%(11/34),胃癌组显著高于后两组(P=0.000),而后两组间无显著性差异(P=0.067);hMLH1蛋白表达率分别为73.3%(126/172)、57.6%(87/151)和41.2%(14/34),胃癌组显著高于后两组(P=0.000),而后两组间无显著性差异(P=0.082)。hMSH2和hMLH1蛋白表达率与胃癌患者的性别、年龄及胃癌分化程度均无显著相关关系。胃癌、癌旁胃粘膜和非癌患者胃粘膜hMSH2蛋白细胞核表达率分别为70.0%(84/120)、58.7%(44/75)和36.4%(4/11),细胞浆表达率分别为30.0%(36/120)、41.3%(31/75)和63.6%(7/11);胃癌、癌旁胃粘膜、非癌患者胃粘膜中,hMSH2蛋白细胞核表达率逐渐降低,而细胞浆表达率则逐渐增高(r=0.161,P=0.020)。三种不同胃粘膜间,hMLH1蛋白在细胞内表达位置无显著性差异(P=0.878)。结论:同时检测胃粘膜错配修复蛋白hMSH2和hMLH1的表达及其细胞内表达位置可能有助于胃癌的早期预警。Objective： To investigate the role and significance of hMSH2 and hMLHI protein expression in gastric carcinogenesis. Methods： We collected 172 samples of gastric carcinoma （GCs）, 151 samples of mucosa surrounding gastrie cancer （SMs） and 34 samples of gastric mucosa from non- cancer patients （NGMs）. Immunohistochemistry was employed to detect hMSH2 and hMLH1 protein expression, and Chi-square test and Spearman correlation analysis were used to analyze the resuhs. Results： In GCs, SMs and NGMs, the expression rates of hMSH2 protein were 69.8% （120/172）, 49. 7% （75/151） and 32.4% （11/34）, respectively （GC vs. SM and NCM, P=0.000; SM vs. NGM, P=0. 067）; those of hMLH1 protein were 73.3% （126/172）, 57.6% （87/151） and 41.2% （14/34）, respectively （GC vs. SM and NGM, P=0.000; SM vs. NGM, P=0.082）. The rates of hMSH2 and hMLH1 protein expression were not significantly correlated with the age or gender of the GC patients or with the differentiation of GC. In GCs, SMs and NGMs, hMSH2 protein positive expression rates were 70.0% （84/ 120）, 58.7% （44/75） and 36.4% （4/11）, respectively, in the nucleus, and 30.0% （36/120）, 41.3% （31/ 75）, 63.6% （7/11）, respectively, in the cytoplasm. No significant difference was found for the location of hMLH1 protein expression. Conclusion： Detection of hMSH2 and hMLH1 proteins in gastric mucosa and analysis of the location of their intracellular expression may be helpful for early diagnosis of gastric carcinoma.

关 键 词：HMSH2 HMLH1 胃癌 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]