Distribution and clonality of peripheral blood TCR Vα subfamily T cells in patients with acute promyelocytic leukemia  

APL患者外周血TCR Vα亚家族T细胞分布和增殖特点(英文)

作　　者：Dongzhi Cen Yangqiu Li Shaohua Chen Lijian Yang Zhi Yu 

机构地区：[1]Institute of Hematology, Medical College, Jinan University, Guangzhou 510632, China

出　　处：《The Chinese-German Journal of Clinical Oncology》2007年第6期591-593,共3页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from National Natural Science Foundation of China (No. 39870358);Natural Science Foundation of Guangdong Province (No. 2005B50301016).

摘　　要：Objective： To investigate the distribution and clonality of TCR Va subfamily T cells in patients with acute promyelocytic leukemia （APL）. Methods： The complementary determining region 3 （CDR3） of TCR Va 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identity the clonality of T cells by GeneScan technique. Results： One to seven of TCR Va subfamilies could be detected in peripheral blood T cells from 9 cases with APL, the frequent expression of Va subfamilies predominated in Vα3 and Va19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Va3, Va26 or Va27 （3 out of 8 cases）. However, almost all Va subfamilies with polyclonal expansion could be detected in peripheral blood T cells from 10 cases of normal individuals. Conclusion： Remarkable skew distribution and clonal expansion of TCR Va subfamilies T cells is the common feature in patients with APL. Clonal expansion of T cells might reflect a response in host to APL cell associated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.

关 键 词：acute promyelocytic leukemia （APL） TCR Va gene CLONALITY 

分 类 号：R73[医药卫生—肿瘤]