高效液相色谱法测定因泼来特注射液中盐酸普鲁卡因和咖啡因的含量  

Simultaneous Determination of Procaine Hydrochloride and Caffeine in Impletol Injection with HPLC

作　　者：林芳[1] 闵敏[1] 贺国芳[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院药学部,武汉430030

出　　处：《医药导报》2007年第7期804-806,共3页Herald of Medicine

摘　　要：目的建立因泼来特注射液中盐酸普鲁卡因和咖啡因的含量测定方法。方法用高效液相色谱（HPLC）法,Hypersil ODS C18色谱柱（250 mm×4.6 mm,5μm）,乙腈-水-三乙胺（13：87：0.02）（稀醋酸调节pH值为3.9）为流动相,流速：0.8 mL·min^-1,检测波长：273 nm。结果盐酸普鲁卡因在5.29～52.90μg·mL^-1范围内线性关系良好,r=0.9999,平均回收率为99.3%,RSD为1.32%;咖啡因在3.78～37.80μg·mL^-1范围内线性关系良好,r=0.9999,平均回收率为98.8%,RSD为1.26%。结论该法简洁,快速,准确,可同时测定两物质的含量。Objective To establish HPLC assay for the determination of procaine hydrochloride and caffeine in impletol injection. Methods The HPLC was conducted on a Hypersil ODS C18 column（250 mm× 4.6 ram, 5 μm） with acetonitrile- water-triethylamine （pH adjusted to 3.9 with dilute acentic acid ） （ 13 ： 87 ： 0.02 ） as mobile phase. The flow rate was 0.8 mL · min ^-1 and detection wavelength was at 273 nm. Results There was a good linear correlation within a range of 5.29-52.90μg · mL^-1 of procaine hydrochloride. The average recovery of procaine hydrochloride was 99.3% （ RSD was 1,32% ） , and there was a good linear correlation within a range of 3, 78-37.80 μg · mL^-1 of caffeine, the average recovery of caffeine was 98.8% （ RSD was 1.26% ）. Conclusion The method is shown to be simple and accurate.

关 键 词：因泼来特注射液 普鲁卡因 盐酸 咖啡因 色谱法 高压液相 

分 类 号：R971[医药卫生—药品] R927.2[医药卫生—药学]