高效液相离子对色谱法测定蒜氨酸的含量及有关物质  

Ion-pairing HPLC determination of alliin and its related substance

作　　者：顾洁[1] 袁耀佐[1] 刘达[1] 杭太俊[1] 张正行[1] 陈坚[2] 

机构地区：[1]中国药科大学药物分析室,南京210009 [2]新疆医科大学药学院药物分析教研室,乌鲁木齐830054

出　　处：《药物分析杂志》2007年第12期1917-1919,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相离子对色谱法测定蒜氨酸的含量。方法:采用 Lichrospher C_(18)色谱柱(4.6 mm×150mm,5μm),流动相为甲醇-磷酸盐缓冲液(0.28%磷酸二氢钾-0.2%庚烷磺酸钠溶液,用85%磷酸调节 pH 为2.1)(10:90),流速为1 mL·min^(-1),紫外214 nm 波长检测,柱温为30℃。结果:蒜氨酸及其有关物质色谱保留时间适宜,分离良好;蒜氨酸在1.0～250μg·mL^(-1)范围内,线性关系良好(r>0.999),日内、日间精密度良好(RSD<1.5%,n=5)。结论:本方法可准确、快速地测定蒜氨酸含量及有关物质检查。Objective： A specific ion - pairing HPLC method was developed for the determination of alliin. Methods： Analysis was carried out on a Lichrospher C18 column（4.6 mm × 150 mm,5μm） with the mixture of methanol and phosphate buffer solution （0.28% KH2PO4 - 0.20% CHs （ CH2 ） 6 SOs Na, pH adjusted to 2.1 with 85% Hs PO4 ） （ 10 ：90） as mobile phase and the flow rate was 1.0 mL .min^-1 and UV detection at 214 nm. Results：The retention and resolution of alliin with its related compounds were good. And linear calibration was found for alliin within the range of 1.0-250 μg . mL^-1. The within- day and between- day precision were good with RSD less than 1.5% （n = 5 ）. Conclusion： The method is simple, accurate and suitable both for assay and related substances test of alliin.

关 键 词：蒜氨酸 有关物质 含量 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]