超声提取-分光光度法测定断血流总皂苷含量  

Determination of total saponins in Clinopodium herb from varieces sources by ultrasonic extraction and spectrophotometry

作　　者：昝丽霞[1] 

机构地区：[1]陕西理工学院生物科学与工程学院,陕西汉中723001

出　　处：《西北大学学报（自然科学版）》2007年第6期1016-1018,共3页Journal of Northwest University（Natural Science Edition）

基　　金：教育部高校博士学科专项科研基金资助项目(20060697014);陕西省教育厅专项科研计划基金资助项目(06JK329);陕西理工学院专项科研基金资助项目(SLGQD0514)

摘　　要：目的对比研究回流法和超声法提取并测定断血流中的总皂苷。方法采用分光光度法直接测定不同产地断血流皂苷的含量。结果断血流皂苷在48．8-488μg范围内有良好线性关系（r=0．9994），总皂苷平均回收率为102．40％，RSD为1．01％（n=3）。结论超声提取法具有简便、快速的特点，分光光度法测定结果准确可靠，重复性好，可用于对药材进行质量控制。Aim To study reflux extraction and ultrasonic extraction methods which were used to extract and determine Clinopodiside in contrast. Methods Using Spectrophotometry to determine the content of total saponins in Clinopodium herb from different places. Results The standard curve of Clinopodiside determined by the Spectrophotometric method was linear in the range of 48.8～ 488 μg （ r = 0. 999 4）, the average recovery of total saponins was 102. 40%, RSD was 1.01% （n =3）. Conclusion Ultrasonic extraction method is simple, effective. Spectrophotometry is reliable, accurate and suitable for the quality control of Clinopodium herb.

关 键 词：断血流 总皂苷 超声提取 分光光度法 

分 类 号：R917[医药卫生—药物分析学]