三氧化二砷抑制人胆囊癌细胞生长及对细胞周期的影响  

Effect of Arsenic Trioxide on the Growth and Cell Cycle of Human Gallbladder Carcinoma Cell

作　　者：凌跃新[1] 艾志龙[1] 锁涛[1] 童赛雄[1] 沈振斌[1] 

机构地区：[1]复旦大学附属中山医院外科,上海200032

出　　处：《中国临床医学》2007年第6期820-821,共2页Chinese Journal of Clinical Medicine

基　　金：复旦大学青年科学基金(项目编号2006135129)

摘　　要：目的:观察三氧化二砷(As_2O_3)对人胆囊癌细胞体内外生长的抑制作用及对细胞周期的影响。方法:不同浓度As_2O_3作用于人胆囊癌GBC细胞,用MTT法检测细胞生长情况,流式细胞仪检测细胞周期分布;20只BALB/c裸鼠皮下接种GBC细胞,随机分为0.9%氯化钠(NS)组(n=10)和As_2O_3组(n=10)。腹腔注射0.9%氯化钠0.2ml·只~1(NS组)或As_2O_3 10 mg·kg^(-1)(As_2O_3组),每周2次,连续7周,测量肿瘤的体积。结果:As_2O_3能抑制体内、外GBC细胞生长.体外实验中As_2O_3作用呈时间、剂量效应关系;流式细胞仪检测发现As_2O_3可将增殖过程中的GBC细胞阻滞于G1期。结论:As_2O_3能明显抑制体内外人胆囊癌GBC细胞的生长及引起G1期阻滞。Objective：To observe the inhibitory effect of As2O3 on the growth of human gallbladder carcinoma in vivo and in vitro and the alteration of cell cycle. Methods： Human gallbladder carcinoma cells（GBC） were cultured with different concentrations of As2O3, the proliferative activity of cells was detected by MTT methods,and the cell cycle status were carried out by flow cytometry（FCM）. Twenty BALB/c nude mice, inoculated with the GBC ceils subcutaneously, were randomly divided into nature saline（NS） and As2O3 groups. 0.2ml NS or 10mg As2O3/kg was injected biweekly into the abdominal cavity of every mouse in NS group or As2O3 group for seven weeks. The tumor size was measured. Results： As2O3 may inhibit the growth of human gallbladder carcinoma in vivo and in vitro, and in a time- and dose dependent manner in vitro. GBC ceils treated with As2O3 were arrested in G1 phase. Conclusion：As2O3 can significantly inhibit the growth of human gallbladder carcinoma in vivo and in vitro as well as induce the G1 phase cell cycle arrest in vitro.

关 键 词：三氧化二砷 人胆囊癌 生长 细胞周期 

分 类 号：R735.8[医药卫生—肿瘤]