大蒜新素对巨细胞病毒感染小鼠细胞因子TGF-β_1的影响  

Effects of Allitridin on TGF-β_1 in Murine Cytomegalovirus Infection

作　　者：杨助锋[1] 方峰[1] 舒赛男[1] 朱单丹[1] 李亚男[1] 李革[1] 董永绥[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030

出　　处：《医药导报》2008年第1期11-14,共4页Herald of Medicine

基　　金：国家自然科学基金项目资助(基金编号:30572345)

摘　　要：目的探讨大蒜新素对全身播散型小鼠巨细胞病毒（MCMV）感染模型鼠转化生长因子β1（TGF—β1）基因转录和蛋白表达的影响。方法建立MCMV全身播散型感染BALB／c小鼠模型，将60只模型鼠随机分为安慰药组和大蒜新素（25mg·kg^-1·d^-1）治疗组，并设60只同期模拟感染小鼠为对照，随机分为模拟对照组和大蒜新素对照组，每组30只。于药物治疗后1，3，7，14和28d获取血清和脾细胞（体外培养刺激物为灭活MCMV抗原）。用双抗体夹心EUSA法检测血清和脾细胞培养上清TGF-β1水平；用逆转录-聚合酶链反应（RT—PCR）法检测脾细胞TGF—β1 mRNA表达强度。结果安慰药组在治疗后3d血清TGF—β1水平即显著上升达高峰，随后逐渐下降，但至28d仍明显高于模拟对照组；其脾细胞TGF-β1 mRNA和蛋白表达变化一致，均在治疗后3d明显增高，7d达高峰，28d回落到稍高于模拟对照组水平。与安慰药组比较，大蒜新素治疗组3d后血清和脾细胞培养上清TGF-β1水平均有降低，以治疗后3～7d差异最为显著；而脾细胞TGF-β1 mRNA表达与大蒜新素对照组相近。结论MCMV在急性感染期可诱导免疫抑制因子TGF—β1基因转录和蛋白表达明显增高，而大蒜新素治疗可完全阻遏病毒诱导的脾细胞TGF—β1基因转录，并明显降低模型鼠血清和脾细胞TGF-β1表达水平.Objective To study the effects of allitridin on the transcription and expression of TGF-β1 gene in the mice model with disseminative infection of murine cytomegalovirus （MCMV）. Methods Sixty BALB/c mice were inoculated with MCMV Smith strains for establishing the model of disseminative infection, and randomly allocated into two groups： placebotreated group and allitridin-treated group with a dose of 25 mg · kg^-1 · d^-1 Another 60 mock-infected mice served as controls and were randomly allocated into allitridin-treated controls and mock-infected controls. The mice were sacrificed for extraction of serum and splenocytes at 1 d, 3 d, 7 d, 14 d and 28 d after treatment, respectively. Inactive MCMV antigens were used as the stimulants for spleen cell culture in vitro. TGF-β1 levels in the serum and culture supernatant of splenocytes were measured by double-antibody sandwich ELISA and TGF-β1 mRNA levels in the splenocytes were detected by RT-PCR assay. Results In placebo-treated group, the TGF-β1 level in serum reached the peak at the 3 d, then went down gradually and was significantly higher than that of mock-infected controls at 28 d. The changes in TGF-β1 mRNA and protein expression in the splenocytes showed similar change, with significant increase at 3 d, going to peak at 7 d, returning to the level a bit higher than that of mockinfected group at 28 d. Comparing with placebo-treated group, TGF-β1 levels both in serum and splenocytes＇supernatant were reduced after 3 days of treatment in allitridin-treated group, showing most significant difference from 3 d to 7 d, meanwhile, TGF-β1 mRNA levels were nearly similar to the those of placebo-treated group. Conclusion MCMV induce a higher transcription and expression of TGF-β1 gene in the acute phase of infection. However, allitfidin can completely inhibit the transcription of TGF-β1 gene and significantly reduce TGF-β1 expressing levels in the serum and splenocytes＇supernatant induced by MCMV.

关 键 词：大蒜新素 巨细胞病毒 TGF—β1 

分 类 号：R966[医药卫生—药理学]