Anticancer effect of aloe-emodin on cervical cancer cells involves G_2/M arrest and induction of differentiation  

作　　者：Jun-ming GUO Bing-xiu XIAO Qiong LIU Shun ZHANG Dong-hai LIU Zhao-hui GONG 

机构地区：[1]Ningbo University School of Medicine, Ningbo 315211, China [2]Affiliated Hospital (Ningbo No 2 Hospital), Ningbo University School of Medicine, Ningbo 315010, China

出　　处：《Acta Pharmacologica Sinica》2007年第12期1991-1995,共5页中国药理学报（英文版）

基　　金：the Ningbo Natural Science Foundation (No 2006A610047);the "151 Personal Training Project" of Zhejiang Province, China;the "Famous Professor Training Project" of Ningbo, China;the "Key Subjects Fund of Ningbo University (No XK0614052);the KC Wong Magna Fund at Ningbo Univer-sity.

摘　　要：Aim： The aim of this study was to investigate the effects of aloe-emodin, a natural compound from the root and rhizome of Rheum palmatum, on the growth of hu- man cervical cancer cells, HeLa. Methods： HeLa cells were treated with various concentrations of aloe-emodin for 1-5 d, and cell growth was measured by 3-（4, 5- dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide assay. The long-term growth effect was investigated by crystal violet assay. The distributions of the cell cycle and apoptosis were analyzed by flow cytometry. The alkaline phos- phatase （ALP） activity was analyzed by a chemical analyzer. Finally, Western blotting was used to indicate the abundant changes of protein kinase C （PKC）, c- myc, cyclins, cyclin-dependent kinases （CDK）, and proliferating cell nuclear anti- gen （PCNA）. Results： Aloe-emodin inhibited the growth of HeLa cells in a dose- dependent manner at concentrations ranging between 2.5 and 40 0rnol/L. The flow cytometric analysis showed that HeLa cells were arrested at the G2/M phase. This effect was associated with the decrease in cyclin A and CDK2, and the increase in cyclin B 1 and CDK1. More importantly, the ALP activity was found to be increased by aloe-emodin treatment, and accompanied by the inhibition of PCNA expression. In addition, aloe-emodin suppressed the expression of PKCα and c-myc. Conclusion： These findings provide a possible mechanistic explana- tion for the growth inhibitory effect of aloe-emodin on HeLa, which includes cell cycle arrest and inducing differentiation.Aim： The aim of this study was to investigate the effects of aloe-emodin, a natural compound from the root and rhizome of Rheum palmatum, on the growth of hu- man cervical cancer cells, HeLa. Methods： HeLa cells were treated with various concentrations of aloe-emodin for 1-5 d, and cell growth was measured by 3-（4, 5- dimethylthiazol-2-yl）-2, 5-diphenyl tetrazolium bromide assay. The long-term growth effect was investigated by crystal violet assay. The distributions of the cell cycle and apoptosis were analyzed by flow cytometry. The alkaline phos- phatase （ALP） activity was analyzed by a chemical analyzer. Finally, Western blotting was used to indicate the abundant changes of protein kinase C （PKC）, c- myc, cyclins, cyclin-dependent kinases （CDK）, and proliferating cell nuclear anti- gen （PCNA）. Results： Aloe-emodin inhibited the growth of HeLa cells in a dose- dependent manner at concentrations ranging between 2.5 and 40 0rnol/L. The flow cytometric analysis showed that HeLa cells were arrested at the G2/M phase. This effect was associated with the decrease in cyclin A and CDK2, and the increase in cyclin B 1 and CDK1. More importantly, the ALP activity was found to be increased by aloe-emodin treatment, and accompanied by the inhibition of PCNA expression. In addition, aloe-emodin suppressed the expression of PKCα and c-myc. Conclusion： These findings provide a possible mechanistic explana- tion for the growth inhibitory effect of aloe-emodin on HeLa, which includes cell cycle arrest and inducing differentiation.

关 键 词：HeLa cells cell cycle ALOE-EMODIN alkalinephosphatase C-MYC 

分 类 号：R96[医药卫生—药理学]