儿童口腔放线菌与儿童龋的关系初探  

Study of the relationship between oral Actinomyces and childhood caries

作　　者：杨燃[1] 邹静[2] 李继遥[3] 

机构地区：[1]四川大学口腔疾病研究国家重点实验室,四川成都610041 [2]四川大学华西口腔医院儿童口腔科,四川成都610041 [3]四川大学华西口腔医院牙体牙髓病科,四川成都610041

出　　处：《华西口腔医学杂志》2007年第6期568-570,共3页West China Journal of Stomatology

基　　金：国家"十五"科技攻关资助项目(2004BA720A24)

摘　　要：目的检测儿童牙面龈上菌斑中的放线菌,初步探讨口腔放线菌与儿童龋病发生的关系。方法选择40例3～5岁儿童为研究对象,其中无龋组和龋敏感组各20例。用无菌牙签收集乳牙表面3个不同部位的龈上菌斑。龋敏感组收集完整面、白垩斑和龋洞内(牙本质层)的菌斑;无龋组收集唇面、邻面和!面沟裂的菌斑。提取菌斑的细菌DNA,用放线菌特异性引物进行聚合酶链反应,对其电泳条带进行记录分析。结果无龋组放线菌检出率为100%,龋敏感组为95%,二者间无统计学差异(P>0.05)。内氏放线菌、戈氏放线菌、龋齿放线菌、衣氏放线菌和黏性放线菌在两组儿童的菌斑中均有检出,前3种放线菌在无龋组的检出率高于龋敏感组,二者间有统计学差异(P<0.05)。2组儿童3个检测部位间的检出率无统计学差异(P>0.05)。结论放线菌是儿童乳牙龈上菌斑中的主要定植细菌,可能是有益菌;其检出情况与乳牙检测部位的关系不大。Objective Actinomyces are members of the indigenous oral microbiota which have been implicated in root caries,but their role in childhood caries initiation and progression is not well-understood.The distribution of Actinomyces in supragingival plaque on the primary teeth and the relationship between oral Actinomyces spp.and childhood caries primarily were investigated.Methods Forty children aged from three to five years old were divided into two groups.Twenty children were caries-free and twenty were caries-susceptible.Dental plaque samples from different places of the teeth were collected with sterile toothpick and polymerase chain reaction was performed with specific primers which were designed for this research.Results Actinomyces spp.were detected 100% in the caries-free group,while 95% in the caries-susceptible group.There was no significant differences between the two groups（P〉0.05）.Actinomyces naeslundii,Actinomyces odontolyticus,Actinomyces gerencseriae,Actinomyces israelii and Actinomyces viscosus all could be detected in both groups.The prevalence of Actinomyces naeslundii,Actinomyces gerencseriae and Actinomyces odontolyticus were higher in caries-free group than those in caries-susceptible group（P〈0.05）,while the prevalence of Actinomyces israelii and Actinomyces viscosus had no significant difference between the two groups（P〉0.05）.Conclusion Actinomyces is the main colonized bacteria in the supragingival plaque of children.The prevalence of the Actinomyces spp.may have a negative relationship with the childhood caries,and has nothing to do with different places of the teeth.

关 键 词：放线菌 聚合酶链反应 龋齿 儿童 

分 类 号：R780.2[医药卫生—口腔医学]