膀胱癌Survivin和VEGF的表达与血管生成关系的研究  

Study of relationship between angiogenesis in bladder cancer and expression of survivin and VEGF

作　　者：赵长林[1] 殷江霞[1] 刘贤奎[2] 徐惠绵[3] 

机构地区：[1]大连大学附属新华医院肿瘤中心,辽宁大连116021 [2]沈阳中国医科大学附属第一医院泌尿外科,110001 [3]沈阳中国医科大学附属第一医院肿瘤科,110001

出　　处：《临床肿瘤学杂志》2007年第12期902-905,共4页Chinese Clinical Oncology

摘　　要：目的:探讨膀胱癌组织中凋亡抑制基因存活素(Survivin)和血管内皮生长因子(VEGF)的表达与膀胱癌血管生成的关系。方法:应用免疫组化S-P法检测69例膀胱癌组织和8例正常膀胱粘膜组织(对照组)中Survivin和VEGF的表达,同时分析不同Survivin和VEGF表达状态下膀胱癌组织微血管密度(MVD)的变化。结果:膀胱癌组和对照组Survivin阳性率分别为71.0%(49/69)和0(0/8),二组比较差异亦有统计学意义(χ2=11.31,P<0.01);膀胱癌组和对照组VEGF阳性率分别为66.7%(46/69)和0(0/8),二组比较差异有统计学意义(χ2=10.62,P<0.01);膀胱癌组织中Survivin和VEGF的表达呈正相关(r=0.785,P<0.01)。将膀胱癌分为4组,A组(n=39)为Survivin+/VEGF+,B组(n=13)为Survivin-/VEGF-,C组(n=7)为Survivin-/VEGF+,D组(n=10)为Survivin+/VEGF-。4组MVD分别为46.69±11.28、30.72±5.10、36.18±8.62、34.47±7.98,A组MVD显著高于其他3组(P均<0.01)。结论:膀胱癌中Survivin可能通过信号传导网络参与调控VEGF的表达而促进血管生成,导致肿瘤恶性进展。Objective：To study the relationship between expression of survivin and vascular endothelial growth factor （VEGF） and angiogenesis in bladder cancer. Methods：By immunohistochemical S-P method, epressions of survivin and VEGF were examined in 69 bladder cancer samples and 8chronic cystitis samples （control group）; and the microvessel density （MVD） was analyzed among different expression states of survivin and VEGF in bladder cancer tissues.Results：The positive rates of survivin in bladder cancer was 71%（49/69） which was significant higer （χ^2=11.31,P〈0.01） than that in control group 0%（0/8）. The positive rates of VEGF in bladder cancer was 66.7%（46/69） which was significant higer （χ^2=10.62,P〈0.01） than that in control group 0%（0/8）. There was a positive correlation between survivin and VEGF expression （r=0.785, P〈0.01）. The bladder cancer group was subdivided into 4 groups： survivin-negative and VEGF-negative cases as groupA （n=39）, survivin-negative and VEGF-negative cases as groupB （n=13）, survivin-positive and VEGF-negative cases as group C（n=7）, survivin-positive and VEGF-negative cases as groupD（n=10）. The meas MVD of groups A, B, C, and D were 46.69±11.28, 30.72±5.10, 36.18±8.62 and 34.47±7.98, respectively. The mean of MVD in group A was significantly higher than other three groups（P〈0.01）. Conclusion：Expression of survivin gene in bladder cancer may promote angiogenesis through increasing in VEGF expression, and thus induce tumor progression.

关 键 词：膀胱肿瘤 存活素 血管内皮生长因子 微血管密度 

分 类 号：R737.14[医药卫生—肿瘤]