栀子苷、黄芩苷、华蟾酥毒基对体外培养的B16恶性黑素瘤细胞细胞增殖的抑制作用  

Inhibition of Proliferation in B16 Melanoma Cells by Jasminoidin,Baicalin and C Inobufagin

作　　者：李雅琳[1] 焦振山[1] 张玉环[1] 刘栋[1] 

机构地区：[1]天津市长征医院,天津300120

出　　处：《中国中西医结合皮肤性病学杂志》2007年第4期206-209,共4页Chinese Journal of Dermatovenereology of Integrated Traditional and Western Medicine

摘　　要：目的观察栀子苷、黄芩苷、吴茱萸内酯、穿心莲内酯、华蟾酥毒基等5种中药单体成分抗恶性黑素瘤的活性。方法以系列浓度的上述5种药物作用体外培养的B16恶性黑素瘤细胞72h,顺铂为对照,MTT法测定细胞增殖抑制率。结果在5种中药单体中,栀子苷、黄芩苷、华蟾酥毒基对体外培养的B16恶性黑素瘤细胞增殖有抑制作用。其中栀子苷对细胞增殖的抑制率呈量效关系,最大抑制率达62.9%;黄芩苷仅在最高浓度时(80μg/mL)对细胞增殖有抑制作用,抑制率为42.8%;华蟾酥毒基浓度≥20μg/mL时表现出对细胞增殖的抑制作用,但无明显量效关系,最大抑制率仅为24.1%(40μg/mL);80μg/mL的栀子苷对细胞增殖的抑制作用与2μg/mL的顺铂无明显差异;穿心莲内酯、吴茱萸内酯则对细胞增殖无明显影响。结论栀子苷、黄芩苷、华蟾酥毒基具有抗恶性黑素瘤的作用,可以做为治疗恶性黑素瘤的备选药物。Objective To investigate the antitumor activities of several natural product compounds in B16 melanoma cells in vitro, Methods Jasminoidin, baicalin, c inobufagin, andrographolide and limonin were incubated with cultured B16 melanoma cells at serial concentrations for 72 h, Inhibition of proliferation in cells were tested by MTT assay, Results Jasminoidin, baicalin and c inobufagin induced cell growth inhibition whereas andrographolide and limonin had no significant effects on cell prohferation. Jasminoidin inhibited proliferation of cells in a dose-dependent manner and the maximum inhibit rate was 62,9% at 80 μg/mL. Baicalin inhibited cell growth only at the highest concentration （80 μg/mL）with the 42,8% suppression of proliferation. C inobufagin induced cell growth at the concentration 20 μg/mL or higher with the maximum inhibit rate 24,1%. No significant different in suppression of proliferation was observed between 80 μg/mL jasminoidin and 2 μg/mL cisplatin（control）. Conclusion Jasminoidin, baicalin and c inobufagin induced cell growth in B16 melanoma cells, which could be candidate drugs for malignant melanoma therapy.

关 键 词：中药 用效单体 恶性黑素瘤 B16恶性黑素瘤细胞 化学疗法 

分 类 号：R739.5[医药卫生—肿瘤]