Functional Identification of the Stable Transfection C5aR Cell Line Molt-4  

作　　者：Chunmei Zhang Ruonan Xu Jianan Wang Gencheng Han Guojiang Chen Renxi Wang Huawei Wei Beifen Shen Yuanfang Ma Yan Li 

机构地区：[1]Institute of Basic Medical Sciences, Beijing, China [2]Laboratory of Cellular and Molecular Immunology, Henan University, Kaifeng, China

出　　处：《Cellular & Molecular Immunology》2007年第6期461-465,共5页中国免疫学杂志（英文版）

基　　金：supported by the grants from the National Key Basic Research Program of China(2007CB512406);the National Natural Science Foundation of China(30571732);National"863"Fund Grant of China(2006AA02A254).

摘　　要：The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Gcxi and Gtz16. C5aR is mostly expressed on neutrophils, macrophages and endothelial cells. C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage. Considering the limitation of collection of human peripheral blood neutrophils and their short half life, the stably transfected cell line for studying the biological effects of C5aR is needed. In this study, we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR. Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERKI/2 phosphorylation, Ca＋＋ influx. This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR. Cellular ＆ Molecular Immunology.The complement C5 anaphylatoxin receptor is a member of the seven transmembrane-spanning G protein-coupled receptor superfamily that signals through Gcxi and Gtz16. C5aR is mostly expressed on neutrophils, macrophages and endothelial cells. C5a and C5aR interaction plays an important role in numerous biological effects such as in vivo cytokine storm which results in inflammatory damage. Considering the limitation of collection of human peripheral blood neutrophils and their short half life, the stably transfected cell line for studying the biological effects of C5aR is needed. In this study, we transfected C5aR gene into Molt-4 cell line and examined the function of ectopic C5aR. Our results showed stable expression of the C5aR in Molt-4 cell line and their interaction with human C5a induced ERKI/2 phosphorylation, Ca＋＋ influx. This stable transfected cell line may provide a useful tool for studying signal pathways related to C5a and C5aR interplay and antibody development specific for C5aR. Cellular ＆ Molecular Immunology.

关 键 词：COMPLEMENT C5A C5AR MOLT-4 

分 类 号：R3[医药卫生—基础医学]