姜黄素诱导人慢粒白血病K562细胞凋亡与线粒体途径的关系  

Induction of apoptosis by curcumin in K562 cells involves mitochondrion pathway

作　　者：吴丽贤[1] 许建华[2] 黄秀旺[1] 张昆仲[1] 温彩霞[1] 陈元仲 

机构地区：[1]福建医科大学药理系,福建福州350004 [2]福建医科大学临床药理研究所,福建福州350004 [3]福建省血液研究所,福建福州350004

出　　处：《世界肿瘤杂志》2007年第4期248-252,共5页Tumour Journal of the World

基　　金：国家自然科学基金（No30171158,30472187）资助：福建省资助省属高校项目（No2005K048）资助

摘　　要：目的研究姜黄素（Cur）对慢性粒细胞白血病（CML）细胞株K562的作用，并且探讨该作用与线粒体凋亡途径的关系。方法应用MTT法检测Cur对细胞增殖的影响，AO／EB荧光染色法、细胞光度术、DNA凝胶电泳等方法观察细胞凋亡，分光光度法检测Caspase-3的活性，用蛋白免疫印迹法检测细胞色素C的含量。结果Cur对K562细胞抑制作用呈量效、时效关系；Cur对人正常骨髓单个核细胞与K562细胞之间有一定的选择性，对K562细胞的敏感性约为人正常骨髓单个核细胞的5倍，在对K562细胞抑制超过95％时，对人正常骨髓有核细胞几无毒性。Cur5．0，10．00g／mL作用24h，可诱导K562细胞凋亡，促进细胞色素C释放，激活caspase-3。结论Cur可通过线粒体途径诱导人慢性粒细胞白血病K562细胞凋亡。Objective To test effects of curcumin （Cur） on K562 cells, and reveal the relationship between Cur-induced apoptosis and mitochondrion pathway. Methods MTI was used to examine the proliferation inhibited by Cur. The typical morphological changes of apoptosis were observed by AO/EB examined by fluorescence microscopy. Quantitate apoptotic cells with subdiploid DNA content was measured by flow cytometry. The activities of Caspase-3 were analyzed by spectrophotometry. The amounts of cytochrome C in cytosolic and S-100 fraction were tested by Western blot. Results Cur was a potent inhibitor of proliferation of K562 cells with low toxicity to normal marrow mononuclear cells. Cur induced cytosolic accumulation of cytochrome c and activities of caspase-3, triggering apoptosis of K562 cells. Conclusions Cur was able to induce the apoptosis of K562 cells by mitochondrion pathway.

关 键 词：姜黄素 K562 慢性粒细胞白血病 细胞色素C caspase-3 

分 类 号：R733.7[医药卫生—肿瘤] R73-34[医药卫生—临床医学]