放射人员人类着色性干皮病基因D多态性与染色体损伤易感性的关系  

Association of genetic polymorphism of XPD with chromosomal damage in workers exposed to radia- tion

作　　者：范雪云[1] 金玉兰[1] 姚三巧[1] 白玉萍[1] 吴旭梅[2] 任大伟[3] 彭健[3] 

机构地区：[1]华北煤炭医学院劳动卫生与环境卫生学学科,唐山063000 [2]唐山市卫生监督所 [3]华北煤炭医学院,唐山01级预防本科生063000

出　　处：《中华劳动卫生职业病杂志》2007年第12期718-721,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：河北省科技攻关课题(052761431)

摘　　要：目的探讨放射人员人类着色性干皮病基因D(XPD)多态性与染色体损伤易感性的关系。方法选择从事放射工作工龄大于1年、出现染色体损伤的182人为病例组,采用1:1配对设计,从同样射线暴露的人员中选择与病例同性别、同民族、年龄相差≤5岁、与病例在同一单位且同工作岗位、工龄相差≤1年、无染色体损伤和血象异常的人为对照。微量全血培养法制备染色体标本,观察、记录染色体畸变率。采用多聚酶链反应-限制性片段长度多态性分析技术(PCR-RFLP)检测XPD基因型。结果病例组751位点野生基因型和156位点突变基因型频率高于对照组,差异有统计学意义(P<0.05):312位点各基因型在两组间分布的差异无统计学意义(P>0.05)。病例组751A和156A等位基因频率高于对照组,差异有统计学意义(P<0.05);312位点等位基因在两组间分布的差异无统计学意义(P>0.05)。结论携带751野生基因型的个体暴露辐射后易发生染色体损伤。XPD 156突变基因型在辐射致染色体损伤过程中可能是一种危险因素。同时携带751AA和156(CA或AA)基因型个体暴露射线后,发生染色体损伤的概率更大。未发现XPD 312位点与辐射致染色体损伤有关联。Objective To explore association genetic polymorphism of XPD with chromosomal damage in workers exposed to radiation. Methods 182 workers exposed to radiation for at least one year with chromosomal damage were selected as cases based on a general health examination for all workers exposed to radiation in Tangshan city. The control group without chromosomal damage was matched to case by age （within 5 years）, sex, work unit, type of exposed to radiation, cumulate serve length （within 1 year） according to 1：1. The micro whole blood cuhivation was used for the chromosome analysis. The chromosome aberration type and rate were observed and counted. The polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used to examine the genotype of three XPD loci （751,312 and 156）. Results The frequency of XPD 751 AA in cases was higher than that in controls（P〈0.05）. The frequency of 751 allele in case group was statistically higher than that in the control groups（P〈0.05）. No statistical difference was found in the frequencies of XPD 312 genotype and allele between the case and control group （P〉0.05）. 156 mutant gene type in case group was higher than that in the control groups. The frequency of 156 A allele in case group were higher than that of the control groups （P〈0.05）. The frequency of genotype with both 751AA and 156CA or 751AA and 156AA was higher in cases than that of controls （P〈0.05）. Conclusion XPD 751AA genotype is a possible risk factor for radiation-inducod chromosomal damage. XPD 156 mutant gene type is a possible risk factor for radiation-induced chromosomal damage. Individuals with both XPD 751AA and 156 （CA＋AA） genotypes are susceptible to radiation-induced chromosomal damage. No association of XPD 312 polymorphism with radiation-induced chromosomal damage is found.

关 键 词：辐射 电离 基因 多态性 单核苷酸 DNA损伤 疾病遗传易感性 

分 类 号：R144[医药卫生—公共卫生与预防医学]