聚乙烯醇(PVA)降解菌的筛选、鉴定和降解特性研究  

Identification and Degradation Characteristic of a Degradation Strain for Poly(Vinyl Alcohol)

作　　者：钱庆荣[1] 肖良建[1] 陈艺兰[1] 肖荔人[1] 陈庆华[1] 

机构地区：[1]福建师范大学化学与材料学院福建省改性塑料技术开发基地,福建福州350007

出　　处：《中国塑料》2007年第12期85-87,共3页China Plastics

基　　金：教育部"新世纪优秀人才支持计划"(NCET-04-0614);国家科技支撑计划(2006BAE03806-03);重大专项专题项目(2007HZ08040043);福建省自然科学基金(D0510016)资助

摘　　要：在有聚乙烯醇(PVA)污染的环境中分离到一株能降解PVA的放线菌。该菌能在以PVA为惟一碳源的无机盐培养基中生长。采用16S rDNA序列分析法,并结合常规生理生化鉴定方法对该菌进行鉴定。16S rDNA序列分析结果表明,该菌与委内瑞拉链霉菌(Streptomyces venezuelae)、砖红链霉菌(Streptomyces lateritius)和脱叶链霉菌(Streptomyces exfoliatus)有最高同源性,达到99%;通过进一步的菌株培养特征及生理生化鉴定表明该菌株与Strep- tomyces venezuelae最接近。因此,初步判定该菌株属于Streptomyces venezuelae,并将其命名为Streptomyces sp.PVA。A poly （vinyl alcohol）（PVA） biodegrading strain was isolated from nature. The strain could grow in the culture media containing PVA as the only carbon source. 16S rDNA analyses and normal methods of physiological and biochemical identification were used in order to identify the strain. The 16S rDNA analyses showed that the strain had the highest comparability with Streptomyces venezuelae, Streptomyces lateritius, and Streptomyces exfoliates, coming to 99 %. From further physiological and biochemical identification we drew the primary conclusion that the strain belonged to the Streptomyces in the classification. And we named it as Streptomyces sp. PVA.

关 键 词：聚乙烯醇 生物降解 委内瑞拉链霉菌 

分 类 号：X172[环境科学与工程—环境科学]