乳腺癌3种耐药基因、p53蛋白、雌和孕激素受体的表达及相关性研究  

Expression of three kinds drug-resistant gene,p53 protein, estrogen and progesterone receptor in breast cancer and their correlation

作　　者：廖海涛[1] 韦义萍[1] 覃洪[1] 罗虹[2] 黄彬[1] 赵刚[1] 陈细珍[1] 

机构地区：[1]广西医科大学护理学院,南宁市530021 [2]广西医科大学第一附属医院,南宁市530021

出　　处：《广西医学》2007年第12期1849-1851,共3页Guangxi Medical Journal

基　　金：广西医疗卫生科研课题(桂卫Z2004152)

摘　　要：目的研究多药耐药基因P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶π(GST-π)和DNA拓扑异构酶Ⅱ(TopoⅡ)、p53蛋白、雌激素受体(ER)和孕激素受体(PR)在乳腺癌的表达及其相关性。方法采用免疫组织化学SABC法,检测119例乳腺癌标本P-gp、GST-π、TopoⅡ、p53、ER和PR的表达。结果P-gp、GST-π、TopoⅡ的表达率分别为52.1%、66.4%和66.4%,p53、ER、PR的表达率分别为56.3%、42.0%和41.2%;乳腺癌中有淋巴结转移组的p53表达率高于无淋巴结转移组(P<0.05),淋巴结转移组的GST-π、ER表达率低于无淋巴结转移组(P<0.05);GST-π、TopoⅡ与p53间表达呈正相关(P<0.05);P-gp、GST-π与ER间表达呈负相关(P<0.05)。结论p53基因与GST-π、TopoⅡ存在协同表达的关系,GST-π、ER阴性是某些乳腺癌对化疗药物不敏感,导致内分泌治疗失败及影响预后的因素。Objective To study the expression of the multidrug-resistant gene P-glycoprotein （ P-gp）, the glutathione-S-transterase -π （GST-π） and DNA topoisomerase Ⅱ （Topo Ⅱ）, p53 protein,estrogen receptor （ER） and progesterone receptor （PR） on breast cancer and their correlation. Methods The specimens obtained from 119 cases of breast cancer were stained, by immunohistochemistry SABC methods for P-gp, GST-π, Topo Ⅱ ,p53,ER and the PR expression. Results The rate of P-gp, GST-π,Topo Ⅱ expression was 52.1% ,66.4% and 66.4% ,the rate of p53 ,ER,PR expression was 56.3% ,42.0% and 41.2% ,respectively. The p53 expression rate of lymphatic metastasis group was higher than that of non-lymphatic metastasis group （P 〈0.05） ,GST-π and ER expression rate of lymphatic metastasis group were lower than those of the non-lymphatic metastasis group （ P 〈 0. 05 ）. There was a positive correlation between the expression of GST-π,Topo Ⅱ and p53 gene （P 〈 0.05 ）, and there was a negative correlation between the expression of P-gp, GST-π and ER （P 〈 0.05）. Condusion The expression of GST-π,Topo Ⅱ and p53 has a close correlation. Breast cancer is insensitive to the chemethempy while the GST-π,ER is negative,leading to the failure of endocrine therapy.

关 键 词：乳腺癌 耐药基因P-糖蛋白 p53 雌激素受体 孕激素受体 谷胱甘肽-S-转移酶Π DNA拓扑异构酶Ⅱ 

分 类 号：R737.9[医药卫生—肿瘤]