儿茶素对环磷酰胺诱导小鼠迟发性超敏反应的影响  

Effects of catechin on cyclophosphamide-induced delayed-type hypersensitivity in mice

作　　者：杨宇清[1] 魏伟[1] 

机构地区：[1]安徽医科大学临床药理研究所安徽省高校省级抗炎免疫药理学重点实验室安徽省高校抗炎免疫药物工程技术研究中心安徽省中药研究与开发重点实验室,合肥230032

出　　处：《中国新药杂志》2007年第23期1940-1943,1949,共5页Chinese Journal of New Drugs

基　　金：国家自然科学基金资助项目(30572356);安徽省自然科学基金(050430903);安徽省科技攻关计划项目(06013133B);安徽高校首批科技创新团队基金(2005TD003)

摘　　要：目的：研究儿茶素对环磷酰胺诱导小鼠迟发性超敏反应的影响。方法：以2,4-二硝基氯苯（DNCB）诱导小鼠迟发性超敏反应（DTH）,致敏后立即腹腔注射（ip）环磷酰胺（Cy）125 mg.kg^-1和致敏前3 d ip Cy 250mg.kg^-1分别诱导小鼠降低和增强的DTH反应,用耳肿胀度、胸腺指数和脾指数作为主要检测指标。以ConA诱导胸腺T淋巴细胞,观察不同剂量或浓度的儿茶素体内外对胸腺T淋巴细胞增殖和IL-2生成的影响。MTT法检测胸腺T淋巴细胞增殖,ConA诱导的胸腺T淋巴细胞培养上清中IL-2活性检测采用活化的小鼠胸腺细胞MTT比色法。结果：于致敏前3 d灌胃儿茶素30,60,120 mg.kg^-1.d^-1,连续7 d,在Cy诱导的DTH反应降低小鼠模型,儿茶素在60,120 mg.kg-1剂量可显著上调其低下的耳肿胀度、胸腺和脾指数,并上调胸腺T淋巴细胞增殖反应以及产生的IL-2水平;在Cy诱导的DTH反应增强小鼠模型,儿茶素60,120 mg.kg^-1可显著下调其增高的耳肿胀、胸腺和脾指数,儿茶素30,60,120 mg.kg-1可显著抑制其增强的胸腺T淋巴细胞增殖反应以及产生的IL-2水平。儿茶素体外在25~200 mg.L^-1浓度时能增强Cy诱导的DTH反应降低的小鼠胸腺T淋巴细胞增殖和IL-2水平;儿茶素在12.5~200 mg.L^-1浓度时能降低Cy诱导的DTH反应增强的小鼠胸腺T淋巴细胞增殖和IL-2水平。结论：儿茶素对小鼠的细胞免疫具有调节作用。Objective： To study the modulatory effects of catechin on cyclophosphamide （Cy）induced delayed-type hypersensitivity （DTH） in mice. Methods： DTH was induced by 2,4-dinitrochlorobenzene （DNCB） in mice. The suppressed DTH was developed by Cy （125 mg.kg-1, ip） simultaneously with DNCB sensitization, and the enhanced DTH by Cy （250 mg·kg^-1 , ip） 3 days before the sensitization. Ear swelling, thymus index and spleen index were used as evaluating indicators. Thymocyte proliferation was induced by concanavalin A （ConA） to determine the effects of catechin at various doses or concentrations on the lymphocyte proliferation and secretion of interleukin-2 in vivo or vitro. Thymocyte proliferation was determined by MTT reduction assay, and IL-2 activity was measured by testing its ability to support ConA-induced thymocyte proliferation. Results： In Cy-suppressed DTH mice, pre-treatment with catechin （60 and 120 mg·kg^-1, ig, once daily for 7 consecutive days starting from 3 days before DTH） significantly enhanced the ear swelling, thymus index, spleen index, thymocyte proliferation and IL-2 level. In Cy-potentiated DTH mice, catechin （60 and 120 mg·kg^-1） significantly attenuated the ear swelling, thymus index and spleen index ; catechin （30, 60 and 120 mg·kg^-1 ） decreased thymocyte proliferation and IL-2 level. Catechin （25 ~ 200 mg·kg^-1） significantly increased in vitro proliferation and IL-2 level in thymocytes from Cysuppressed DTH mice; while it （12.5 -200 mg·kg^-1） reduced the proliferation and IL-2 level in thymocytes from Cy-potentiated DTH mice. Conclusion： Catechin possesses a modulatory effect on cell immunity of mice.

关 键 词：儿茶素 迟发性超敏反应 T淋巴细胞 白介素-2 

分 类 号：R965.1[医药卫生—药理学] R979.5[医药卫生—药学]