瘦素受体基因Lys109Arg多态性与慢性肝病的关系  

An investigation of the relationship between receptor gene Lys109Arg polymorphisms and patients with chronic liver disease

作　　者：丁百静[1] 朴云峰[1] 关英慧[1] 李静[1] 祝尔健[1] 王凯[1] 

机构地区：[1]吉林大学第一医院消化科

出　　处：《临床内科杂志》2007年第12期840-842,共3页Journal of Clinical Internal Medicine

摘　　要：目的探讨瘦素受体基因Lys109Arg多态性与慢性肝病及血清瘦素水平的关系。方法采用聚合酶链反应及限制性片段长度多态性方法(PCR-RFLP)测定150例慢性肝病患者和89例对照组的瘦素受体Lys109Arg多态性的基因型,同时检测两组血清瘦素水平。结果慢性肝病组与对照组的瘦素受体Lys109Arg基因型频率及等位基因频率比较差异无显著性(P>0.05);慢性肝病患者中慢性肝炎、肝硬化间基因型频率及等位基因频率比较差异无显著性(P>0.05);慢性肝病患者中Arg109Arg基因型携带者的空腹血清瘦素水平较Lys109Arg基因型低(P<0.05)。结论瘦素受体Lys109Arg基因多态性与慢性肝病患者的空腹血清瘦素水平有关。Objective To investigate leptin receptor gene polymorphisms and their correlations with leptin levels and chronic liver disease. Methods 150 patients with chronic liver disease and 89 healthy individuals were studied. The leptin receptor gene polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism assay （PCR-RFLP）. Serum levels of leptin were determined by enzymelinked immunoabsorbent assay （ ELISA ）. Allele frequencies in patients with chronic liver disease and controls were assessed and their correlations with serum leptin levels were analyzed. Results The genotype frequency of Lysl09Arg polymorphism in leptin receptor gene had no significant difference between patients with chronic liver disease and controls （ P 〉 0.05 ）. The genotype frequency of Lys109Arg polymorphism in leptin receptor gene had no significant difference between patients with chron- ic hepatitis and cirrhosis （ P 〉 0.05）. The mean fasting serum leptin levels in chronic liver disease with Lysl09Arg genotype[ （3.04 ±0.36）ng/ml] was higher than those with Arg109Arg genotype [ （2.86 ± 0.43 ）ng/ml] ,the difference was significant （P 〈 0.05 ）. Conclusions The Lys109Arg polymorphism in leptin receptor gene is associated with fasting serum leptin levels in patients with chronic liver disease.

关 键 词：肝病 慢性疾病 瘦素 基因多态现象 遗传学 

分 类 号：R575[医药卫生—消化系统]