Survivin反义寡核苷酸增加A375细胞对顺铂的敏感性  

Oligonucleotide sensitizes A375 cells to cisplatin

作　　者：王梅[1] 王晓丽[2] 王冰[3] 谭升顺[1] 

机构地区：[1]西安交通大学医学院第二附属医院皮肤科,陕西西安710004 [2]西安交通大学医学院第一附属医院肿瘤科,陕西西安710061 [3]西安交通大学医学院病理系,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2007年第6期642-644,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科技攻关基金资助项目(No.2003K10-G10)

摘　　要：目的研究Survivin反义寡核苷酸对人恶性黑色素瘤A375细胞顺铂治疗敏感性的影响。方法台盼蓝计数观察Survivin反义寡核苷酸和顺铂对A375细胞增殖的抑制作用。结果5μmol/L反义寡核苷酸与0.5 mg/L顺铂共同作用于A375细胞72 h,细胞生长抑制率为61.3%,高于单因素作用组,组间比较差异具有统计学意义(P<0.001)。用反义寡核苷酸预处理细胞24 h后加入顺铂对A375细胞的生长抑制率为68.0%,高于同时用药组,但组间差异无统计学意义。结论反义寡核苷酸和顺铂联合用药对A375细胞生存具有协同抑制效应,即Survivin反义寡核苷酸可增强A375细胞对顺铂治疗的敏感性。Objective To investigate whether targeting survivin has the potential to sensitize A375 cells to chemotherapy. Methods By trypan blue exclusion assays we observed A375 cell proliferation inhibition induced by survivin antisense oligonucleotide （ASODN） and cisplatin. Results A375 cells treated with a combination of 5 μmol/L ASODN and 0.5 mg/L cisplatin, and approximately 61.3% of the cells showed signs of cell death 72 h after the start of transfection trypan blue exclusion assays. Compared to treatments with either oligonucleotide or cisplatin alone, there were significant differences among the groups（P〈0. 001）. Cells were pre-treated 24 h with 5 μmol/L ASODN before combination treatment, and 68.0% of the cells showed signs of cell death, but there were not significant differences between combination treated group and pre-treated group. Conclusion A combination treatment with ASODN and cisplatin possesses synergy in inhibiting A375 cell proliferation, indicating that oligonucleotide sensitizes A375 cells to cisplatin therapy.

关 键 词：SURVIVIN 顺铂 恶性黑色素瘤 细胞凋亡 

分 类 号：R739.5[医药卫生—肿瘤]