P^(27)儿童髓母细胞瘤中的表达变化及意义  

THE SIGNIFICANCE AND EXPRESSION OF P^(27) IN CHILDHOOD MEDULLOBLASTOMA

作　　者：陈礼刚 李定君[1] 明扬[1] 刘洛同[1] 董劲虎[1] 杨福兵[1] 张苓[1] 

机构地区：[1]泸州医学院附属医院神经外科,四川泸州646000

出　　处：《泸州医学院学报》2007年第6期473-475,共3页Journal of Luzhou Medical College

摘　　要：目的:检测P^(27)蛋白在儿童髓母细胞瘤中的表达情况,探讨P^(27)与预后的关系。方法:收集84例经手术病理证实井获随访的儿童髓母细胞瘤临床病理资料,男46例,女38例,年龄6～12岁,按术后生存期3、5、10年,分为A、B、C三组,每组病例数各为42例、27例和15例。应用免疫组化和激光共聚焦显微术检测肿瘤P^(27)蛋白的表达情况。结果:84例儿童髓母细胞瘤中,P^(27)阳性表达率为22.35%～98.63%,平均为62.12%。A、B、C三组P^(27)阳性表达率分别为32.41%,64.46%,96.53%(P<0.01)。激光共聚焦显微术显示P^(27)在肿瘤细胞质中平均阳性表达率为40.48%,而A、B、C三组阳性表达率分别为87.42%、70.08%和48.02%,三者两两比较,差异均有统计学意义(P<0.01)。Cox比例风险模型分析显示,P^(27)低表达,P^(27)在细胞质中阳性表达是儿童髓母细胞瘤预后不良因素(相对危险度和95%CI分别是10.3及2.3～6.7和3.9及1.6～4.1)。结论:P^(27)在儿童髓母细胞瘤中有不同程度的降低,P^(27)低表达及在细胞质中高表达是儿童髓母细胞瘤预后的危险因素。Objective：To detect the expression and localization of P^27 in childhood medulloblastoma and its prognostic value. Methods：84 patients undergoing microneurosurgical procedures for children medullobastoma which had been proved surgically and pathologically were retrospectively analyzed. Male 46 cases, female 38 cases, age from 6 to 12 years. According to the survival period of 3,5 and 10 yoars in fellow up were, patients divided into group A,B and C respectively. There were 42,27 and 15 cases respectively. The expression of P^27 was studied by immunohistochemistry and confocal technique. The data were analyzed by Cox regression analysis. Results：Tile positive rate of P^27 expression was 62.12% in average, and it was 32.41%, 64.46%, 96.53% respectively in group A,B,C （P〈0.01）. P^27 was verified with the cytoplasm localization by confocal techniqne in childhood medulloblastoma and the expression was 40.48% in average, and was 87%,70%,48% in group A,B,C respetively （P〈0.01）. The lower expression and the cytoplasm localization of P^27 were showed as independent risk factors for children medulloblastoma by cox proportional hazard model analysis, with the relative risk of 10.3 （95% CI2.3-6.7） and 3.9 （95%CI1.6-4.1） respectively. Conclusion：P27 was decreased in childhood medulloblastonma, and the lower expression and the cytoplasw localization of p27 were of independent prognostic value.

关 键 词：儿童 髓母细胞瘤 P^27 预后 

分 类 号：R730.23[医药卫生—肿瘤]