白细胞介素—13对人红白细胞白血病细胞株的作用“英文”  

Effect of interleukin-13 on the growth of human erythroleukemia cell line

作　　者：石小玉[1] 李文林[2] 奚晓东[3] 韩忠朝 JacquesP.Caen 

机构地区：[1]江西医学院形态中心实验室,南昌330006 [2]江西医学院病理生理学教研室 [3]苏州医学院血液研究室 [4]法国巴黎血管,血液研究所

出　　处：《中国病理生理杂志》1997年第4期337-340,共4页Chinese Journal of Pathophysiology

摘　　要：观察了人重组白细胞介素-13(IL-13)在体外对人红白细胞白血病细胞株(HEL)的作用。该细胞株是巨核细胞白细胞株。培养液中加入或不加入IL-13,HEL细胞培养4天后,计数细胞,然后直接免疫荧光染色,流式细胞仪检测糖蛋白(GP)Ⅱb/Ⅲa表达。为研究IL-13对HEL细胞增殖的影响,细胞培养3天后,用免疫细胞法检测掺入HEL细胞DNA中的5-溴-2′-脱氧尿苷酸(5-Brdu)。结果表明:IL-13使GP Ⅱb/Ⅲa表达增加(与对照组比较,P<0.05),也能促进细胞增殖(与对照组比较,P<0.05)。以上结果提示IL-13对HEL细胞的增殖和分化有显著的调节作用。In this study we investigated the effect of recombinant human interleukin-13 (IL-13) on the growth of human erythroleukemia cell line(HEL)-a human megakaryobiastic leukemia cell line. HEL cells were cultured in the presence or absence of IL-13. After incubation for 4 days, the cells were first counted and then stained with a direct immunofiuorescence method for flow cytometric analysis of the expression of glycoprotein(GP) Ⅱ b/Ⅲ a. In order to examine the effect of IL-13 on the proliferation of HEL cells, the immunocyte assay was used for the detection of 5-bromo-2'-deoxyurid:ine (5-Brdu) incorporated into the DNA of HEL cells after they had been cultured for 3 days. We found that the expression of GP Ⅱ b/Ⅲ a on IL-13 treated HEL cells was increased to a significant degree (P<0.05, compared with control). Besides, IL-13 enhanced significantly the proliferation of HEL cells(P<0.05, compared with control). Our data suggest that IL-13 may play an important regulatory role in the proliferation and differentiation of HEL cells.

关 键 词：白细胞介素-13 巨核细胞 糖蛋白 白血病 

分 类 号：R733.705[医药卫生—肿瘤]