动脉粥样硬化性脑梗塞患者载脂蛋白B基因的MspⅠ限制性片段长度多态性研究  

THE MspⅠ RESTRICTION FRAGMENT LENGTH POLYMORPHISMS OF APOLIPOPROTEIN B (Apo B) GENE IN CHINESE ATHEROSCLEROTIC CEREBRAL INFARCTION PATIENTS

作　　者：赵若智[1,2,3,4] 刘锡民[1,2,3,4] 蔡转 阮旭中[1,2,3,4] 杨明山 沈斌章[1,2,3,4] 刘春杰 

机构地区：[1]北京医科大学人民医院神经内科 [2]同济医科大学同济医院神经内科 [3]华中农业大学作物遗传改良国家重点实验室 [4]北京右安医院

出　　处：《中华医学遗传学杂志》1997年第4期227-230,共4页Chinese Journal of Medical Genetics

摘　　要：为了解动脉粥样硬化性脑梗塞（ａｔｈｅｒｏｓｃｌｅｒｏｔｉｃｃｅｒｅｂｒａｌｉｎｆａｒｃｔｉｏｎ，ＡＣＩ）与载脂蛋白代谢异常的关系，利用ＡｐｏＢｃＤＮＡ探针（ＬＢ１．５）检测了５４名ＡＣＩ患者和６２名正常对照者的载脂蛋白Ｂ（ＡｐｏＢ）基因ＭｓｐⅠ限制性片段长度多态性（ＲＦＬＰ）等位基因频率。结果显示，１１６份标本共出现２．３５ｋｂ（Ｍ１）和２．６０ｋｂ（Ｍ２）两条杂交片段。Ｍ２等位基因频率在ＡＣＩ（０．１５７）和对照者（０．０５６）之间存在明显差异（χ２＝７．１７，Ｐ＜０．０１）。Ｍ２基因的检出和基因频率的获得为进一步揭示ＡｐｏＢ基因区域在ＡＣＩ的脂类代谢中的作用提供了一个很好的遗传标记。还就不同基因型与血脂、脂蛋白水平的关系进行了讨论。We have investigated MspⅠ allele frequencies of restriction fragment length polymorphisms (RFLP) of the ApoB gene in 54 Chinese atherosclerotic cerebral infarction (ACI) patients and 62 healthy subjects, with the ApoB cDNA probe (LB1.5). The result showed that 2.35 kb (M 1) and 2.60 kb (M 2) hybridization fragment were found in 116 samples. The rare allele (M 2) was seen more frequently in patients than in controls (0.157 vs 0.056, χ 2=7.17, P<0.01) . The M 2 polymorphic site may be a useful genetic marker for revealing ApoB gene roles in the lipid metabolism of ACI. In addition, we have investigated serum lipid and lipoprotein levels in relation to ApoB genotypes (MspⅠ) in the Chinese controls and ACI patients.

关 键 词：脑梗塞 载脂蛋白B 多态性 动脉粥样硬化 

分 类 号：R743.330.2[医药卫生—神经病学与精神病学]