蚯蚓对细菌降解土壤中菲的作用  

Role of earthworm in degradation of soil phenanthrene by Pseudomonas putida

作　　者：胡淼[1] 陈欢[1] 田蕾[1] 胡锋[1] 魏正贵[1] 李辉信[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095

出　　处：《应用生态学报》2008年第1期218-222,共5页Chinese Journal of Applied Ecology

基　　金：国家环境保护总局项目(江苏省土壤污染调查);南京农业大学SRT资助项目(0607B17).

摘　　要：通过30d室内培养试验,分别研究了接种蚯蚓(E)、细菌(B)以及同时接种细菌和蚯蚓(BE)对土壤中菲降解的影响.结果表明:在土壤中菲的初始污染浓度为50mg.kg-1的条件下,各处理间菲的降解率差异显著,其降解率的大小顺序依次为:BE>B>E>CK(对照);在150mg.kg-1菲的初始污染浓度下,BE处理中菲的降解率高达98.86%,显著高于CK和E处理.B处理中细菌的双加氧酶活性在3种菲初始污染浓度下没有显著差异,而BE处理中双加氧酶的活性随着土壤中菲的初始污染浓度的升高而增加.在相同菲污染浓度下BE处理中蚯蚓体内的菲含量明显高于E处理.表明蚯蚓能够通过生物富集作用降低土壤中菲的浓度,而蚯蚓与细菌的相互作用能够进一步促进土壤中菲的降解.A 30-day incubation test was conducted to investigate the effects of treatments earthworm （ E）, bacteria （ Pseudomonas putida） （B） and earthworm-bacteria （BE） on the degradation of soil phenanthrene. The degradation rate of soil phenanthrene at its initial concentration of 50 mg ·kg^-1 was in the sequence of BE 〉 B 〉 E 〉 CK, and that at the concentration of 150 mg ·kg^-1 was 98.86% in BE, being significantly higher than that in CK and E. With the increase of the initial concentration of soil phenanthrene, the bacterial dioxygenase activity almost did not change in B, but increased significantly in BE. Under the same concentration of soil phenanthrene, the phenanthrene content in earthworm was significant higher in BE than in E, suggesting that earthworm could decrease the concentration of soil phenanthrene via its bioaccumulation, and the interaction between earthworm and P. putida could further promote the biodegradation of soil phenanthrene.

关 键 词：蚯蚓 假单胞菌 菲 双加氧酶 

分 类 号：X172[环境科学与工程—环境科学]