戊型肝炎病毒感染动物模型应用于抗-HEV试剂评价的初步研究  

Primary evaluation of anti-HEV diagnostic reagent by experimental infection animal model with hepatitis E virus

作　　者：周诚[1] 黄维金[1] 吴星[1] 蓝海云[1] 辜文洁[1] 黄果勇[2] 张华远[1] 祁自柏[1] 李河民[1] 

机构地区：[1]中国药品生物制品检定所,北京100050 [2]广西壮族自治区疾病预防控制中心

出　　处：《中华流行病学杂志》2008年第1期48-51,共4页Chinese Journal of Epidemiology

摘　　要：目的应用实验室戊型肝炎病毒（HEV）感染恒河猴动物模型对抗-HEV诊断试剂进行初步的评价。方法用1和4基因型的HEV静脉注射分别感染4只恒河猴，检测实验猴的丙氨酸氨基转移酶（ALT）水平，逆转录聚合酶链反应（RT-PCR）方法检测粪便标本中HEVRNA，抗-HEVIgG试剂（GL-IgG，wT-IgG）分别检测实验猴系列血清抗体水平。结果HEV感染的8只实验猴均出现粪便排毒，1和4基因型HEV感染的实验猴分别有1只和2只出现ALT升高，GL-IgG试剂检测1型HEV实验感染猴有2只抗体阳转，4型HEV实验感染猴有1只抗体阳转；而wT-IgG试剂检测1、4型HEV实验感染猴的抗体均阳转。两种试剂检测感染猴窗口期抗体阳转时间相近，GL-IgG试剂抗体阳性持续到12周，wrT-IgG试剂在16周观察期内均阳性。结论两种试剂均可检测出感染实验猴的抗体，但wT-IgG试剂具有检测灵敏度较高的特点。Objective To evaluate anti-HEV diagnostic kits by experimental infecting rhesus monkeys with HEV, Methods Eight rhesus monkeys were infected with genotype 1 and 4 HEV separately. The alanine aminotransferase（ALT） level of all monkeys were detected before and after the process of infection, HEV RNA in stool specimens was tested by reverse transcriptase-polymerase chain reaction （RT-PCR） assay, Anti-HEV IgG in serum was detected by GL-IgG and WT-IgG, Results HEV RNA presented in the stool of all the 8 monkeys after infection. The ALT level of 1 monkey infected with genotype 1 HEV and 2 monkeys infected with genotype 4 HEV appeared abnormally after infection. Tested by GL-IgG,2 of the 4 monkeys infected with genotype 1 HEV and 1 of 4 monkeys infected with genotype 4 HEV seroconverted to anti-HEV IgG, However, when tested by WT-IgG, all the infected monkeys seroconverted to anti-HEV IgG, The anti-HEV IgG tested by WT-IgG was positive during the whole observation period,and the anti-HEV IgG measured by GL-IgG only remained 12 weeks after infection, Detected by GL-IgG and WT-IgG, seropositive conversion of the anti-HEV IgG happened almost at the same time, Conclusion Both GL IgG and WT-IgG could detect the anti-HEV IgG of experimentally infected rhesus monkeys but the WT-IgG had a higher sensitivity for detection of anti-HEV IgG than GL-IgG.

关 键 词：戊型肝炎病毒 动物模型 酶联免疫吸附试验 

分 类 号：R51[医药卫生—内科学] R-332[医药卫生—临床医学]