手性配体高效液相色谱法拆分扁桃酸对映体的热力学性质  

Thermodynamic Studies on Enantiomer Separation of Mandelic Acid by Chiral Ligand-exchange HPLC

作　　者：李桂银[1,2] 黄可龙[1] 蒋玉仁[1] 丁萍[1] 

机构地区：[1]中南大学化学化工学院,长沙410083 [2]湖南科技职业学院轻化工程学院,长沙

出　　处：《应用化学》2008年第1期9-12,共4页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(20376085);湖南省教育厅资助项目

摘　　要：在对称C18高效液相色谱柱上，以L-苯丙氨酸和硫酸铜为手性配体流动相，在温度293～313K范同内，考察了扁桃酸对映体的保留和分离行为。在实验范围内，温度升高对分离不利，随着温度的升高，对映体的保留时间、分离度和选择性因子均减少；用In k’对1／T作图，得Van’t Hoff曲线具有良好的线性关系，相关系数R〉0．99。计算了扁桃酸对映体在色谱柱分离的焓变、焓变差值和熵变差值等热力学参数。在293K〈T〈313K温度范围内，扁桃酸对映体满足｜△S，S△H^0｜〉｜T△S，R△S^0｜，故手性拆分过程为焓控过程。The effect of temperature （293 ～313 K） on chromatographic separation of mandelic acid over a C18 column was investigated with L-phenylalanine-CuSO4 aqueous solution and methanol as the chiral ligand mobile phase. Higher column temperature was found to be disadvantageous to the enantiomer separation. The retention time, resolution and selectivity of the chiral separation were decreased with temperature increase. The Van＇t Hoff plots of the In k＇ versus 1/T of the enantiomers have a good linearity with a correlation coefficient of 0. 99. The thermodynamic functions（△H^0, △S,R△H^0, △S,R△S^0） were calculated, and in the examined temperature range, the enthalpy contribution to the enantiomer transfer energy was found to be more significant than the entropy one. Therefore, the enantiomeric separation is a enthalpy controlling process.

关 键 词：手性配体 扁桃酸 对映体拆分 热力学性质 

分 类 号：O642[理学—物理化学]