氧自由基在血管紧张素Ⅱ诱导ECV304细胞增殖中的作用  

Role of Oxygen Free Racical in the Proliferation of ECV304 Induced by AngⅡ

作　　者：李雄[1] 张旋[2] 王殿华[2] 

机构地区：[1]77206部队60分队卫生所 [2]昆明医学院云南省天然药物药理重点实验室

出　　处：《昆明医学院学报》2007年第6期53-57,共5页Journal of Kunming Medical College

摘　　要：目的探讨氧自由基在血管紧张素Ⅱ诱导ECV304细胞增殖中的作用.方法体外培养人脐静脉内皮细胞株(ECV304),实验分为AngⅡ处理组、NAC干预组和正常对照组.采用改良MTT法、Fenton反应和硝酸酶还原法分别观察AngⅡ处理组、NAC干预组和正常对照组的ECV304细胞的增殖率和细胞产生氧自由基(·OH)的量.结果一定浓度的AngⅡ(0.03125～1μmol/L)作用12 h时ECV304细胞增殖率增加,明显高于正常对照组(P<0.05);AngⅡ可诱导ECV304细胞产生氧自由基(·OH),氧自由基(·OH)的含量与ECV304细胞增殖率呈显著负相关;10 mmol/LNAC可抑制1μmol/LAngⅡ对ECV304细胞的增殖作用,与正常对照组相比无显著性差异(P>0.05).结论AngⅡ可诱导ECV304细胞产生氧自由基(·OH),氧自由基(·OH)含量与AngⅡ呈时间和剂量依赖性;NAC可抑制AngⅡ对ECV304细胞的增殖作用,这可能与减少氧自由基(·OH)的含量有关;氧自由基(·OH)可能是AngⅡ诱导ECV304细胞增殖的主要信号转导分子之一.Objective To explore the role of oxygen free radicals in the proliferation of ECV304 induced by Ang Ⅱ. Methods The lines of human umbilical vein endothelial cell （ECV304） cultured in vivo were divided into three groups which were treated by Ang Ⅱ , Ang Ⅱ ＋N-acetyl-L-cysteine （NAC） , and normal culture medium. First we observed the proliferous effect of ECV304 induced by Ang Ⅱ at different concentration with improved MTT and microscope. Then the contents of oxygen free radicals （·OH） in three groups were detected by spectrophotometer. Results ECV304 incubated with Ang Ⅱ （0.03125 ～1μmol/L） for 12 hours increased the proliferation rate （P 〈 0.05 vs. control group） ; It was significant that the negative correlation between proliferation rate and the content of oxygen free radicals; NAC （10mmol/L） inhibited the proliferation of ECV304 induced by Ang Ⅱ （P 〉 0.05 vs. control group ）. Conclusions ECV304 induced by Ang Ⅱ can produce oxygen free radicals （·OH）, and the contents of oxygen free radicals （· OH） increase with the prolongation of time and the enlargement of dose; Antioxidant NAC can inhibit the proliferation of ECV304 induced by Ang Ⅱ , this effect may be related with reducing the content of oxygen free radicals （·OH） ; oxygen free radicals （·OH） may be one of the major mocleculars which play an important role in the signal transduction of ECV304 proliferation.

关 键 词：N-乙酰半胱氨酸 血管紧张素Ⅱ 氧自由基ECV304细胞 增殖 

分 类 号：R363[医药卫生—病理学]